Simple Plex Human UCH-L1 Cartridge

Name: Simple Plex Human UCH-L1 Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-010947

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-010947

Simple Plex Human UCH-L1 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (10 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL), Cerebrospinal Fluid (10 uL)
Sensitivity:	17.87 pg/mL
Assay Range:	32.77 - 50,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid)
Simple Plex Human UCH-L1 Cartridge Summary:	Simple Plex assay for the detection of human Ubiquitin C-terminal Hydrolase L1 (UCH-L1) in cell culture supernates (CCS), serum, plasma (EDTA/Heparin), and cerebrospinal fluid (CSF).

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human UCH-L1 Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human UCH-L1 Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant UCH-L1.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=2)	106	106-107
Cerebrospinal Fluid (n=4)	107	99-113
EDTA Plasma (n=4)	110	107-112
Heparin Plasma (n=4)	117	104-123
Serum (n=4)	116	109-122

Linearity

Samples containing and/or spiked with high concentrations of UCH-L1were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Examples for Simple Plex Human UCH-L1 Cartridge

	Human UCH-L1 Ella Assay Standard Curve

Preparation and Storage

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: UCH-L1/PGP9.5

Ubiquitin Carboxyl-terminal Esterase L1 (UCH-L1), also known as PGP9.5, is a deubiquitinating enzyme with a predicted molecular weight of 25 kDa. The human protein shares 95% amino acid sequence identity with its mouse and rat orthologs. UCH-L1 is expressed abundantly in neurons, accounting for 1-2% of total soluble proteins in the brain. It localizes primarily to the cytoplasm, but a subpopulation has been shown to be transiently nuclear. UCH-L1 contains two catalytic residues, Cys90 and His161, which are required for isopeptide bond cleavage at the C-terminal glycine residue of Ubiquitin. The levels of free Ubiquitin appear to be partially regulated by UCH-L1 through the hydrolysis of small Ubiquitin chains and the stabilization of monomeric Ubiquitin. Mice lacking functional UCH-L1 show neuronal dysfunction and neurodegeneration, and mutations in this enzyme have been linked to Parkinson's disease, suggesting that it is important for proper central nervous system function. UCH-L1 also likely plays a complex role in cancer. It has been reported to function as an oncogene in lymphoma, colorectal cancer, and nonsmall cell lung carcinoma. In contrast, it is thought to function as a tumor suppressor protein in nasopharyngeal and breast cancers.

Item	Value
Long Name	Ubiquitin C-terminal Hydrolase L1
Entrez Gene IDs	7345 (Human); 22223 (Mouse)
Alternate Names	EC 3.4.19.12, EC 6.-, Neuron cytoplasmic protein 9.5, PARK5, PGP 9.5, PGP9.5, PGP9.5Uch-L1, PGP95, UCH-L1, UCHL1, Ubiquitin thioesterase L1, ubiquitin C-terminal hydrolase, ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase), ubiquitin carboxyl-terminal hydrolase isozyme L1

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Human UCH-L1 Cartridge

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Protocols & Manuals

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