Simple Plex Mouse CXCL2/GRO beta/MIP-2/CINC-3 Cartridge

Name: Simple Plex Mouse CXCL2/GRO beta/MIP-2/CINC-3 Cartridge
Brand: ProteinSimple
SKU: SPCKB-MP-000583

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-MP-000583

Simple Plex Mouse CXCL2/GRO beta/MIP-2/CINC-3 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD09, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Mouse Cell Culture Supernates (500 nL), Mouse Serum (25 uL), Mouse EDTA Plasma (25 uL), Mouse Heparin Plasma (25 uL)
Sensitivity:	0.06 pg/mL
Assay Range:	0.92 - 1,412 pg/mL (Mouse Cell Culture Supernates, Mouse Serum, Mouse EDTA Plasma, Mouse Heparin Plasma)
Simple Plex Mouse CXCL2/GRO beta/MIP-2/CINC-3 Cartridge Summary:	Simple Plex assay for the detection of mouse MIP-2 in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin)

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

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Technical Data
Citations

Product Specifications for Simple Plex Mouse CXCL2/GRO beta/MIP-2/CINC-3 Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Mouse
Specificity	Natural and recombinant mouse MIP-2.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Mouse Cell Culture Supernates, Mouse Serum, Mouse EDTA Plasma, Mouse Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	91	92
Mean (pg/mL)	10.9	475	10.3	463
Standard Deviation	0.994	40.8	1.2	39.3
CV%	9.1	8.6	11.6	8.5

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Mouse EDTA Plasma (n=4)	106	100-115
Mouse Heparin Plasma (n=4)	107	100-116
Mouse Serum (n=4)	104	94-113

Linearity

Samples containing and/or spiked with high concentrations of mouse MIP-2 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data Examples for Simple Plex Mouse CXCL2/GRO beta/MIP-2/CINC-3 Cartridge

	Mouse CXCL2/MIP-2 Simple Plex Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CXCL2/GRO beta/MIP-2/CINC-3

CXCL2/GRO beta, also called MIP-2 in mouse and CINC-3 in rat, is a member of the CXC chemokine family. Human CXCL2/GRO beta is 107 amino acids (aa) in length with a predicted molecular weight of 11 kDa. The mouse and rat orthologs share 70% and 71% aa sequence identity with the human protein, respectively. N-terminal aa 1-4 of CXCL2/GRO beta can be post-translationally cleaved which confers enhanced hematopoietic bioactivity. CXCL2/GRO beta is produced by a variety of cell types including monocytes and macrophages at sites of inflammation and is chemotactic for granulocytes, including neutrophils.

Item	Value
Entrez Gene IDs	2920 (Human); 20310 (Mouse); 114105 (Rat)
Alternate Names	C-X-C Motif Chemokine 2, CINC-2a, CINC-3, CINC3, CXCL2, GRO beta, GRO2, GRO2 oncogene, GROB, Gro-beta, Growth-regulated protein beta, MGSA beta, MGSA-b, MGSA-beta, MIP-2, MIP-2a, MIP2, MIP2-alpha, MIP2A, Macrophage inflammatory protein 2-alpha, SCYB2, chemokine (C-X-C motif) ligand 2, melanoma growth stimulatory activity beta

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Mouse CXCL2/GRO beta/MIP-2/CINC-3 Cartridge

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