Simple Plex Mouse/Rat CCL2/JE/MCP-1 Cartridge

Name: Simple Plex Mouse/Rat CCL2/JE/MCP-1 Cartridge
Brand: ProteinSimple
SKU: SPCKB-MP-000584

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-MP-000584

Simple Plex Mouse/Rat CCL2/JE/MCP-1 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD09, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Mouse Cell Culture Supernates (5 uL), Rat Cell Culture Supernates (1 uL), Mouse Serum (25 uL), Rat Serum (5 uL), Mouse EDTA Plasma (25 uL), Rat EDTA Plasma (25 uL), Mouse Heparin Plasma (25 uL), Rat Heparin Plasma (25 uL), Mouse Cerebrospinal Fluid (25 uL)
Sensitivity:	0.16 pg/mL
Assay Range:	0.71 - 1,090 pg/mL (Mouse Cell Culture Supernates, Rat Cell Culture Supernates, Mouse Serum, Rat Serum, Mouse EDTA Plasma, Rat EDTA Plasma, Mouse Heparin Plasma, Rat Heparin Plasma, Mouse Cerebrospinal Fluid)
Simple Plex Mouse/Rat CCL2/JE/MCP-1 Cartridge Summary:	Simple Plex assay for the detection of mouse/rat MCP-1 in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), and mouse cerebrospinal fluid (CSF)

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

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Technical Data
Citations

Product Specifications for Simple Plex Mouse/Rat CCL2/JE/MCP-1 Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Mouse, Rat
Specificity	Natural and recombinant mouse/rat MCP-1.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Mouse Cell Culture Supernates, Rat Cell Culture Supernates, Mouse Serum, Rat Serum, Mouse EDTA Plasma, Rat EDTA Plasma, Mouse Heparin Plasma, Rat Heparin Plasma, Mouse Cerebrospinal Fluid

	Intra-Assay Precision		Inter-Assay Precision
Sample
n	16	16	54	54
Mean (pg/mL)	331	6.41	313	6.6
Standard Deviation	20.2	0.484	27.4	0.697
CV%	6.1	7.5	8.8	10.6

Linearity

Samples containing and/or spiked with high concentrations of mouse/rat MCP-1 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data Examples for Simple Plex Mouse/Rat CCL2/JE/MCP-1 Cartridge

	Mouse/Rat MCP-1 Simple Plex Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CCL2/JE/MCP-1

CCL2/JE/MCP-1, is a chemokine that binds the receptor CCR2 and induces the chemoattraction of mononuclear cells. It induces the activation of monocytes, NK cells, lymphocytes, and basophils. Additionally, CCL2 promotes Th2 polarization in CD4+ T cells, and CCL2-mediated recruitment of monocytes to sites of inflammation contributes to disease severity in atherosclerosis, multiple sclerosis, and allergic asthma. Endogenous proteolytic trimming of CCL2 at the N-terminus, including the N-terminal pyrrolidone carboxylic acid-modified glutamine, downregulates activity but not receptor binding.

Item	Value
Entrez Gene IDs	6347 (Human); 20296 (Mouse); 24770 (Rat); 403981 (Canine)
Alternate Names	C-C motif chemokine ligand 2, CCL2, GDCF-2, HC11, HSMCR30, MCAF, MCP-1, Mcp1, SCYA2, SMC-CF

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Mouse/Rat CCL2/JE/MCP-1 Cartridge

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