Simple Plex Rat S100B Cartridge

Name: Simple Plex Rat S100B Cartridge
Brand: ProteinSimple
SKU: SPCKC-RP-009923

ProteinSimple, part of Bio-Techne | Catalog # SPCKC-RP-009923

Product Datasheet / COA

Simple Plex Rat S100B assay kit for use on Ella instrument. Contains cartridge, sample diluent SD10, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (10 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Cerebrospinal Fluid (10 uL)
Sensitivity:	0.648 pg/mL
Assay Range:	4.01 - 2,448 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid)
Simple Plex Rat S100B Cartridge Summary:	Simple Plex assay for the detection of rat S100 Calcium Binding Protein B (S100B) in cell culture supernatant (CCS), serum and plasma (EDTA/Heparin) and cerebrospinal fluid (CSF).

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Rat S100B Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Rat S100B Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Rat
Specificity	Natural and recombinant S100B.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	72	71
Mean (pg/mL)	32	1845	23.5	1443
Standard Deviation	2.09	67.3	2.16	115
CV%	6.6	3.6	9.2	8

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=2)	102	93-108
EDTA Plasma (n=5)	120	116-124
Heparin Plasma (n=5)	115	104-125
Serum (n=3)	93	93-95

Linearity

Samples containing and/or spiked with high concentrations of S100B were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Examples for Simple Plex Rat S100B Cartridge

	Rat S100B Ella Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: S100B

S100B belongs to the S100 subgroup of the EF-hand family of calcium binding proteins. It is a homodimer that is expressed primarily in the brain by astrocytes, oligodendrocytes and Schwann cells. S100B has multiple intracellular functions, but can also be secreted from cells to exert extracellular functions. Some of the extracellular functions of S100B may be mediated by RAGE (receptor for advanced glycation end products). Blood levels of S100B can be used to monitor the extent of brain injury and malignant melanoma.

Item	Value
Long Name	S100 Calcium Binding Protein B
Entrez Gene IDs	6285 (Human)
Alternate Names	NEF, S-100 calcium-binding protein, beta chain, 10protein S100-B, S-100 protein beta chain, S-100 protein subunit beta, S100, S100 beta, S100 calcium binding protein B, S100 calcium-binding protein B, S100 calcium-binding protein, beta (neural), S100B, S100beta, beta (neural)

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.