α-Amanitin

Name: α-Amanitin
Brand: Tocris Bioscience
SKU: 4025
Rating: 5 (1 reviews)

Tocris Bioscience, part of Bio-Techne | Catalog # 4025

(1)

Citations (6)

COA / SDS

Inhibitor of RNA polymerase II

Key Product Details

Description:	Inhibitor of RNA polymerase II
Chemical Name:	cyclo[L-Asparaginyl-4-hydroxy-L-proly-(R-4,5-dihydroxy-L-isoleucyl-6-hydroxy-2-mercapto-L-tryptophylglycyl-L-isoleucylglycyl-L-cysteinyl]cyclic (4→8)-sulfide (R)-S-oxide
Purity:	≥95% (HPLC)
Molecular Weight:	918.97

View Full Technical Details for α-Amanitin

Technical Data
Citations / Reviews (1)

Biological Activity for α-Amanitin

α-Amanitin is an inhibitor of RNA polymerase II. Inhibits transcription in eukaryotic cells. Binds and blocks the largest subunits of RNA polymerase II, preventing new ribonucleotides from incorporating into the nascent RNA chain. Potent amatoxin.

Technical Data for α-Amanitin

M. Wt	918.97
Formula	C₃₉H₅₄N₁₀O₁₄S
Storage	Store at -20°C
Purity	≥95% (HPLC)
CAS Number	23109-05-9
PubChem ID	441541
InChi Key	CIORWBWIBBPXCG-JAXJKTSHSA-N
Smiles	O=[C@@](N[C@@](C(N[C@@H](CC2=C(S4=O)NC3=C2C=CC(O)=C3)C(NCC(N[C@]([H])5[C@@H](C)CC)=O)=O)=O)([H])[C@@H](C)[C@@H](O)CO)[C@@H]1C[C@@H](O)CN1C([C@H](CC(N)=O)NC([C@H](C4)NC(CNC5=O)=O)=O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for α-Amanitin

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	water	4.59	5

Preparing Stock Solutions for α-Amanitin

The following data is based on the product molecular weight 918.97.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.05 mM	21.76 mL	108.82 mL	217.63 mL
0.25 mM	4.35 mL	21.76 mL	43.53 mL
0.5 mM	2.18 mL	10.88 mL	21.76 mL
2.5 mM	0.44 mL	2.18 mL	4.35 mL

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Reconstitution Calculator

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References for α-Amanitin

References are publications that support the biological activity of the product.

Raha Close association of RNA polymerase II and many transcription factors with Pol III genes. Proc.Natl.Acad.Sci.
Casse The transcriptional inhibitors, actinomycin D and α-amanitin, activate the HIV-1 promoter and favor phosphorylation of the RNA polymerase II C-terminal domain. J.Biol.Chem. 1999 PMID: 10347161