Anisomycin

Name: Anisomycin
Brand: Tocris Bioscience
SKU: 1290
Rating: 5 (0 reviews)

Tocris Bioscience, part of Bio-Techne | Catalog # 1290

Citations (115)

COA / SDS

JNK, SAPK and p38 activator

Key Product Details

Description:	JNK, SAPK and p38 activator
Chemical Name:	(2R,3S,4S)-2-[(4-Methoxyphenyl)methyl]-3,4-pyrrolidinediol 3-acetate
Purity:	≥98% (HPLC)
Molecular Weight:	265.31

View Full Technical Details for Anisomycin

Technical Data
Citations

Biological Activity for Anisomycin

Anisomycin is a protein synthesis inhibitor (blocks translation). Potent activator of stress-activated protein kinases (JNK/SAPK) and p38 MAP kinase. Acts as a potent signaling agonist to selectively elicit homologous desensitization of immediate early gene induction (c-fos, fosB, c-jun, junB and junD).

Technical Data for Anisomycin

M. Wt	265.31
Formula	C₁₄H₁₉NO₄
Storage	Store at +4°C
Purity	≥98% (HPLC)
CAS Number	22862-76-6
PubChem ID	253602
InChi Key	YKJYKKNCCRKFSL-RDBSUJKOSA-N
Smiles	O[C@H]1CN[C@H](CC2=CC=C(OC)C=C2)[C@@H]1OC(C)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Anisomycin

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	ethanol	13.27	50
	DMSO	26.53	100

Preparing Stock Solutions for Anisomycin

The following data is based on the product molecular weight 265.31.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.77 mL	18.85 mL	37.69 mL
5 mM	0.75 mL	3.77 mL	7.54 mL
10 mM	0.38 mL	1.88 mL	3.77 mL
50 mM	0.08 mL	0.38 mL	0.75 mL

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Reconstitution Calculator

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References for Anisomycin

References are publications that support the biological activity of the product.

Sanchez Role of SAPK/ERK kinase-1 in the stress-activated pathway regulating transcription factor c-Jun. Nature 1994 PMID: 7997269
Kyriakis The stress-activated protein kinase subfamily of c-Jun kinases. Nature 1994 PMID: 8177321
Croons The protein synthesis inhibitor anisomycin induces macrophage apoptosis in rabbit atherosclerotic plaques through p38 mitogen-activated protein kinase. J.Pharmacol.Exp.Ther. 2009 PMID: 19286921
Hazzalin Anisomycin selectively desensitizes signalling components involved in stress kinase activation and fos and jun induction. Mol.Cell.Biol. 1998 PMID: 9528756
Cano Anisomycin-activated protein kinases p45 and p55 but not mitogen-activated protein kinases ERK-1 and -2 are implicated in the induction of c-fos and c-jun. Mol.Cell.Biol. 1994 PMID: 7935449