Belinostat

Name: Belinostat
Brand: Tocris Bioscience
SKU: 7981

Tocris Bioscience, part of Bio-Techne | Catalog # 7981

COA / SDS

Potent pan-HDAC inhibitor

Key Product Details

Description:	Potent pan-HDAC inhibitor
Chemical Name:	(2E)-N-Hydroxy-3-[3-[(phenylamino)sulfonyl]phenyl]-2-propenamide
Purity:	≥98% (HPLC)
Molecular Weight:	318.35

View Full Technical Details for Belinostat

Technical Data
Citations

Biological Activity for Belinostat

Belinostat is a potent pan-HDAC inhibitor (IC₅₀ = 28 nM). Dose-dependently reduces tumor volume in a rat model of glioblastoma and induces 70% and 28% apoptosis in LN-229 and LN-18 glioma cell lines (IC₅₀ values are 210 nM and 300 nM respectively). In H358 cells, Belinostat dose-dependently reduces cell viability and upregulates amino acids and metabolites of the TCA and urea cycle. Also potently inhibits C4 release from stem cell-derived astrocytes and shows antidepressant activity. Belinostat is brain penetrant.

Technical Data for Belinostat

M. Wt	318.35
Formula	C₁₅H₁₄N₂O₄S
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	866323-14-0
PubChem ID	6918638
InChi Key	NCNRHFGMJRPRSK-MDZDMXLPSA-N
Smiles	S(NC1=CC=CC=C1)(=O)(=O)C2=CC(/C=C/C(NO)=O)=CC=C2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Belinostat

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	31.84	100
	ethanol	31.84	100

Preparing Stock Solutions for Belinostat

The following data is based on the product molecular weight 318.35.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.14 mL	15.71 mL	31.41 mL
5 mM	0.63 mL	3.14 mL	6.28 mL
10 mM	0.31 mL	1.57 mL	3.14 mL
50 mM	0.06 mL	0.31 mL	0.63 mL

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References for Belinostat

References are publications that support the biological activity of the product.

Gurbani Assessing treatment response of glioblastoma to an HDAC inhibitor using whole-brain spectroscopic MRI. Tomography 2019 PMID: 30854442
Rapino Small-molecule screen reveals pathways that regulate C4 secretion in stem cell-derived astrocytes. Stem Cell Reports 2023 PMID: 36563689
Kusaczuk Molecular and cellular effects of a novel hydroxamate-based HDAC inhibitor - belinostat - in glioblastoma cell lines: a preliminary report. Invest.New Drugs 2016 PMID: 27468826
Peter Histone deacetylase inhibitor belinostat regulates metabolic reprogramming in killing KRAS-mutant human lung cancer cells. Mol.Carcinog. 2022 PMID: 37144836
Finn Novel sulfonamide derivatives as inhibitors of histone deacetylase. Helv.Chim.Acta