CGP 74514 dihydrochloride

Name: CGP 74514 dihydrochloride
Brand: Tocris Bioscience
SKU: 5472

Tocris Bioscience, part of Bio-Techne | Catalog # 5472

COA / SDS

Potent CDK1 inhibitor

Key Product Details

Description:	Potent CDK1 inhibitor
Chemical Name:	rel-N²-[(1R,2S)-2-Aminocyclohexyl]-N⁶-(3-chlorophenyl)-9-ethyl-9H-purine-2,6-diamine dihydrochloride
Purity:	≥98% (HPLC)
Molecular Weight:	458.82

View Full Technical Details for CGP 74514 dihydrochloride

Technical Data
Citations

Biological Activity for CGP 74514 dihydrochloride

CGP 74514 dihydrochloride is a potent cdk1 inhibitor (IC₅₀ = 25 nM). Reduces Akt phosphorylation and increases mitochondrial damage in leukemia cells in vitro in combination with LY 294002.

Technical Data for CGP 74514 dihydrochloride

M. Wt	458.82
Formula	C₁₉H₂₄ClN₇.2HCl
Storage	Desiccate at RT
Purity	≥98% (HPLC)
PubChem ID	121513864
InChi Key	YFAKNNUHXLCNLG-FZMMWMHASA-N
Smiles	CCN2C1=NC(N[C@@H]4CCCC[C@@H]4N)=NC(NC3=CC(Cl)=CC=C3)=C1N=C2.Cl.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for CGP 74514 dihydrochloride

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	water	22.94	50
	DMSO	45.88	100

Preparing Stock Solutions for CGP 74514 dihydrochloride

The following data is based on the product molecular weight 458.82.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.18 mL	10.90 mL	21.80 mL
5 mM	0.44 mL	2.18 mL	4.36 mL
10 mM	0.22 mL	1.09 mL	2.18 mL
50 mM	0.04 mL	0.22 mL	0.44 mL

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References for CGP 74514 dihydrochloride

References are publications that support the biological activity of the product.

Furet Structure-based design of potent CDK1 inhibitors derived from olomoucine. J.Comput.Aided Mol.Des. 2000 PMID: 10896313
Yu The lethal effects of pharmacological cyclin-dependent kinase inhibitors in human leukemia cells proceed through a phosphatidylinositol 3-kinase/Akt-dependent process. Cancer Res. 2013 PMID: 12702569