dTAG-47

Name: dTAG-47
Brand: Tocris Bioscience
SKU: 7530

Tocris Bioscience, part of Bio-Techne | Catalog # 7530

Citations (3)

COA / SDS

Degrades mutant FKBP12F36V fusion proteins; useful alternative to genetic methods for target validation

Key Product Details

Description:	Degrades mutant FKBP12^F36V fusion proteins; useful alternative to genetic methods for target validation
Chemical Name:	(1R)-3-(3,4-Dimethoxyphenyl)-1-(2-(2-((8-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-5-yl)amino)octyl)amino)-2-oxoethoxy)phenyl)propyl (2S)-1-((S)-2-(3,4,5-trimethoxyphenyl)butanoyl)piperidine-2-carboxylate
Purity:	≥97% (HPLC)
Molecular Weight:	1076.25

View Full Technical Details for dTAG-47

Technical Data
Citations

Biological Activity for dTAG-47

dTAG-47 is a degrader targeting mutant FKBP12^F36V fusion proteins. Comprises a ligand selective for F36V single-point mutated FKBP12, a linker and a cereblon-binding ligand. Application of dTAG-47 induces rapid, reversible and selective degradation of FKBP12^F36V fusion proteins in vitro and in vivo. dTAG is generalizable to a range of fusion proteins; useful as an alternative to genetic methods for target validation. See also dTAG-13 and dTAG-7.

Negative control (Cat. No. 7531) also available.

FKBP12^F36V can be expressed as a fusion with a target protein of interest using genome engineering techniques, via transgene expression or CRISPR-mediated locus-specific knock-in.

Plasmid vectors for the lentiviral expression and CRISPR-mediated knock-in of FKBP12^F36V are available from Addgene.

Licensing Information

Sold under license from Dana-Farber Cancer Institute

Technical Data for dTAG-47

M. Wt	1076.25
Formula	C₅₉H₇₃N₅O₁₄
Storage	Store at -20°C
Purity	≥97% (HPLC)
CAS Number	2265886-81-3
PubChem ID	153280948
InChi Key	OQXAQHQPOQLCAE-ODPQSFPTSA-N
Smiles	CC[C@@H](C1=CC(OC)=C(C(OC)=C1)OC)C(N2CCCC[C@H]2C(O[C@@H](C3=C(C=CC=C3)OCC(NCCCCCCCCNC4=CC=C5C(N(C(C5=C4)=O)C6CCC(NC6=O)=O)=O)=O)CCC7=CC(OC)=C(C=C7)OC)=O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for dTAG-47

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	107.62	100

Preparing Stock Solutions for dTAG-47

The following data is based on the product molecular weight 1076.25.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	0.93 mL	4.65 mL	9.29 mL
5 mM	0.19 mL	0.93 mL	1.86 mL
10 mM	0.09 mL	0.46 mL	0.93 mL
50 mM	0.02 mL	0.09 mL	0.19 mL

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References for dTAG-47

References are publications that support the biological activity of the product.

Layden A protocol for rapid degradation of endogenous transcription factors in mammalian cells and identification of direct regulatory targets. STAR Protoc. 2021 PMID: 34041503
Weintraub YY1 is a structural regulator of enhancer-promoter loops. Cell 2017 PMID: 29224777
Brunetti Mutant NPM1 maintains the leukemic state through HOX expression. Cancer Cell. 2018 PMID: 30205049
Sreekanth Chemogenetic system demonstrates that Cas9 longevity impacts genome editing outcomes. ACS Cent.Sci. 2020 PMID: 33376784
Bryan WDR5 is a conserved regulator of protein synthesis gene expression. Nucleic Acids Res. 2020 PMID: 31996893
Popay MYC regulates ribosome biogenesis and mitochondrial gene expression programs through its interaction with host cell factor-1. Elife 2021 PMID: 33416496