dTAG-Fluorescein

Name: dTAG-Fluorescein
Brand: Tocris Bioscience
SKU: 7892

Tocris Bioscience, part of Bio-Techne | Catalog # 7892

COA / SDS

Fluorescent probe for labeling mutant FKBP12 (F36V) proteins

Key Product Details

Description:	Fluorescent probe for labeling mutant FKBP12 (F36V) proteins
Chemical Name:	(R)-1-(2-(2-((3',6'-Dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthen]-5-yl)amino)-2-oxoethoxy)phenyl)-3-(3,4-dimethoxyphenyl)propyl (S)-1-((S)-2-(3,4,5-trimethoxyphenyl)butanoyl)piperidine-2-carboxylate
Purity:	≥95% (HPLC)
Molecular Weight:	1023.1

View Full Technical Details for dTAG-Fluorescein

Technical Data
Citations

Biological Activity for dTAG-Fluorescein

dTAG-Fluorescein is a fluorescent probe for labeling FKBP12^F36V fusion proteins. Comprises a high-affinity ligand for FKBP12^F36V mutant protein (K_d in the subnanomolar range and 1,000-fold selectivity for the F36V mutant of FKBP12 over wild-type) coupled to 5-amino fluorescein. dTAG-Fluorescein can fluorescently label FKBP12^F36V fusion proteins expressed at different levels in a variety of cell lines.

Optical Data for dTAG-Fluorescein

Emission Color	Green
λ_abs	493 nm
λ_em	514 nm
Extinction Coefficient (ε)	68800 M^-1cm^-1
Closest Laser line	488 nm
Application	Flow cytometry, Confocal microscopy

Technical Data for dTAG-Fluorescein

M. Wt	1023.1
Formula	C₅₈H₅₈N₂O₁₅
Storage	Store at -20°C
Purity	≥95% (HPLC)
InChi Key	ZSRZNOZTAUPBBF-ZSDGZGBPSA-N
Smiles	CC[C@@H](C1=CC(OC)=C(C(OC)=C1)OC)C(N2CCCC[C@H]2C(O[C@@H](C3=C(C=CC=C3)OCC(NC4=CC(C(O5)=O)=C(C=C4)C65C7=C(C=C(C=C7)O)OC8=C6C=CC(O)=C8)=O)CCC9=CC(OC)=C(C=C9)OC)=O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for dTAG-Fluorescein

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	10.23	10

Preparing Stock Solutions for dTAG-Fluorescein

The following data is based on the product molecular weight 1023.1.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.1 mM	9.77 mL	48.87 mL	97.74 mL
0.5 mM	1.95 mL	9.77 mL	19.55 mL
1 mM	0.98 mL	4.89 mL	9.77 mL
5 mM	0.20 mL	0.98 mL	1.95 mL

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Reconstitution Calculator

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References for dTAG-Fluorescein

References are publications that support the biological activity of the product.

Marks A general approach for chemical labeling and rapid, spatially controlled protein inactivation. Proc.Natl.Acad.Sci.U.S.A. 2004 PMID: 15218100
Nabet The dTAG system for immediate and target-specific protein degradation. Nat.Chem.Biol. 2018 PMID: 29581585