Gemcitabine hydrochloride

Name: Gemcitabine hydrochloride
Brand: Tocris Bioscience
SKU: 3259
Rating: 5 (2 reviews)

Tocris Bioscience, part of Bio-Techne | Catalog # 3259

(2)

Citations (26)

COA / SDS

DNA synthesis inhibitor

Key Product Details

Description:	DNA synthesis inhibitor
Chemical Name:	(+)-2'-Deoxy-2',2'-difluorocytidine hydrochloride
Purity:	≥99% (HPLC)
Molecular Weight:	299.66

View Full Technical Details for Gemcitabine hydrochloride

Technical Data
Citations / Reviews (2)

Biological Activity for Gemcitabine hydrochloride

Gemcitabine hydrochloride is a deoxycytidine analog that inhibits DNA synthesis. Metabolized to form gemcitabine triphosphate (dFdCTP) and gemcitabine diphosphate (dFdCDP). dFdCTD inhibits ribonucleotide reductase causing a reduction in cellular nucleotides. dFdCTP is incorporated in DNA resulting in DNA strand termination. Displays antitumor activity in vitro and in vivo. Inhibits replication of MERS-CoV and SARS-CoV in vitro (EC₅₀ values are 1.216 and 4.957 μM, respectively).

Compound Libraries for Gemcitabine hydrochloride

Gemcitabine hydrochloride is also offered as part of the Tocriscreen 2.0 Max, Tocriscreen Antiviral Library and Tocriscreen FDA-Approved Drugs. Find out more about compound libraries available from Tocris.

Technical Data for Gemcitabine hydrochloride

M. Wt	299.66
Formula	C₉H₁₁F₂N₃O₄.HCl
Storage	Desiccate at +4°C
Purity	≥99% (HPLC)
CAS Number	122111-03-9
PubChem ID	60749
InChi Key	OKKDEIYWILRZIA-OSZBKLCCSA-N
Smiles	OC[C@@H]1[C@@H](O)C(F)(F)[C@H](N2C(N=C(N)C=C2)=O)O1.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Gemcitabine hydrochloride

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	water	29.97	100
	DMSO	5.99	20

Preparing Stock Solutions for Gemcitabine hydrochloride

The following data is based on the product molecular weight 299.66.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.34 mL	16.69 mL	33.37 mL
5 mM	0.67 mL	3.34 mL	6.67 mL
10 mM	0.33 mL	1.67 mL	3.34 mL
50 mM	0.07 mL	0.33 mL	0.67 mL

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Reconstitution Calculator

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References for Gemcitabine hydrochloride

References are publications that support the biological activity of the product.

Dyall Repurposing of clinically developed drugs for treatment of Middle East Respiratory Syndrome coronavirus infection. Antimicrob.Agents.Chemother. 2014 PMID: 24841273
Heinemann Gemcitabine: a modulator of intracellular nucleotide and deoxynucleotide metabolism. Semin.Oncol. 1995 PMID: 7481839
Plunkett Preclinical characteristics of gemcit. Anticancer Drugs 1995 PMID: 8718419
Hertel Evaluation of the antitumor activity of gemcit. (2',2'-Difluoro-2'-deoxycytidine). Cancer Res. 1990 PMID: 2364394