JIB 04

Name: JIB 04
Brand: Tocris Bioscience
SKU: 4972

Tocris Bioscience, part of Bio-Techne | Catalog # 4972

Citations (24)

COA / SDS

Pan Jumonji inhibitor; active in vivo

Key Product Details

Description:	Pan Jumonji inhibitor; active in vivo
Chemical Name:	5-Chloro-2-[(E)-2-[phenyl(pyridin-2-yl)methylidene]hydrazin-1-yl]pyridine
Purity:	≥99% (HPLC)
Molecular Weight:	308.76

View Full Technical Details for JIB 04

Technical Data
Citations

Biological Activity for JIB 04

JIB 04 is a pan Jumonji histone demethylase inhibitor (IC₅₀ values are 230, 340, 435, 445, 855 and 1100 nM for JARID1A, JMJD2E, JMJD2B, JMJD2A, JMJD3 and JMJD2C respectively). Exhibits negligible activity at PHD2 and TET1. Selectively blocks cancer cell growth in vitro and diminishes tumor growth in H358 and A549 mouse xenograft models in vivo. Prolongs survival in a mouse model of breast cancer.

Compound Libraries for JIB 04

JIB 04 is also offered as part of the Tocriscreen 2.0 Max and Tocriscreen Epigenetics Library. Find out more about compound libraries available from Tocris.

Technical Data for JIB 04

M. Wt	308.76
Formula	C₁₇H₁₃ClN₄
Storage	Store at +4°C
Purity	≥99% (HPLC)
CAS Number	199596-05-9
PubChem ID	6519698
InChi Key	YHHFKWKMXWRVTJ-OQKWZONESA-N
Smiles	ClC(C=C3)=CN=C3N/N=C(C2=CC=CC=C2)/C1=CC=CC=N1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for JIB 04

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	30.88	100

Preparing Stock Solutions for JIB 04

The following data is based on the product molecular weight 308.76.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.24 mL	16.19 mL	32.39 mL
5 mM	0.65 mL	3.24 mL	6.48 mL
10 mM	0.32 mL	1.62 mL	3.24 mL
50 mM	0.06 mL	0.32 mL	0.65 mL

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References for JIB 04

References are publications that support the biological activity of the product.

Grunicke and Hofmann Azinyl and diazinyl hydrazones derived from aryl N-heteroaryl ketones: synthesis and antiproliferative activity. J. Med. Chem. 1997 PMID: 9435912
Wang A small molecule modulates Jumonji histone demethylase activity and selectively inhibits cancer growth. Nat. Commun. 2013 PMID: 23792809