Nutlin 3a

Name: Nutlin 3a
Brand: Tocris Bioscience
SKU: 6075

Tocris Bioscience, part of Bio-Techne | Catalog # 6075

Citations (16)

COA / SDS

MDM2 antagonist; active enantiomer of Nutlin-3 (Cat. No. 3984)

Key Product Details

Description:	MDM2 antagonist; active enantiomer of Nutlin-3 (Cat. No. 3984)
Chemical Name:	4-[[(4S,5R)-4,5-Bis(4-chlorophenyl)-4,5-dihydro-2-[4-methoxy-2-(1-methylethoxy)phenyl]-1H-imidazol-1-yl]carbonyl]-2-piperazinone
Purity:	≥97% (HPLC)
Molecular Weight:	581.49

View Full Technical Details for Nutlin 3a

Technical Data
Citations

Biological Activity for Nutlin 3a

Nutlin 3a is an MDM2 antagonist (IC₅₀ = 90 nM); inhibits the MDM2-p53 interaction. Active enantiomer of Nutlin-3 (Cat. No. 3984). Induces expression for P53 regulated genes. Suppresses tumor growth in vivo >98% in prostate cancer and osteosarcoma cancer models. Antiproliferative.

Compound Libraries for Nutlin 3a

Nutlin 3a is also offered as part of the Tocriscreen 2.0 Max and Tocriscreen Epigenetics Library. Find out more about compound libraries available from Tocris.

Technical Data for Nutlin 3a

M. Wt	581.49
Formula	C₃₀H₃₀Cl₂N₄O₄
Storage	Store at -20°C
Purity	≥97% (HPLC)
CAS Number	675576-98-4
PubChem ID	11433190
InChi Key	BDUHCSBCVGXTJM-WUFINQPMSA-N
Smiles	ClC1=CC=C([C@H]2[C@H](N(C(N3CC(NCC3)=O)=O)C(C4=CC=C(OC)C=C4OC(C)C)=N2)C5=CC=C(Cl)C=C5)C=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Nutlin 3a

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	58.15	100
	ethanol	58.15	100

Preparing Stock Solutions for Nutlin 3a

The following data is based on the product molecular weight 581.49.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.72 mL	8.60 mL	17.20 mL
5 mM	0.34 mL	1.72 mL	3.44 mL
10 mM	0.17 mL	0.86 mL	1.72 mL
50 mM	0.03 mL	0.17 mL	0.34 mL

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References for Nutlin 3a

References are publications that support the biological activity of the product.

Vassilev In vivo activation of the p53 pathway by small-molecule antagonists of MDM2. Science 2004 PMID: 14704432
Tovar Small-molecule MDM2 antagonists reveal aberrant p53 signaling in cancer: implications for therapy. Proc.Natl.Acad.Sci.USA. 2006 PMID: 16443686