PF 5274857 hydrochloride

Name: PF 5274857 hydrochloride
Brand: Tocris Bioscience
SKU: 5262

Tocris Bioscience, part of Bio-Techne | Catalog # 5262

COA / SDS

High affinity and selective Smoothened (Smo) receptor antagonist

Key Product Details

Description:	High affinity and selective Smoothened (Smo) receptor antagonist
Chemical Name:	1-[4-(5'-Chloro-3,5-dimethyl[2,4'-bipyridin]-2'-yl-1-piperazinyl]-3-(methylsulfonyl)-1-propanone hydrochloride
Purity:	≥98% (HPLC)
Molecular Weight:	473.42

View Full Technical Details for PF 5274857 hydrochloride

Technical Data
Citations

Biological Activity for PF 5274857 hydrochloride

PF 5274857 hydrochloride is a high affinity and selective Smoothened (Smo) receptor antagonist (K_i = 4.6 nM). Exhibits >1000-fold selectivity for Smo over a panel of other receptors, ion channels and enzymes. Inhibits Shh-induced Hedgehog signaling in MEF cells. Induces tumor regression in a medulloblastoma allograft mouse model. Orally available and brain penetrant.

Licensing Information

Sold for research purposes under agreement from Pfizer Inc.

Compound Libraries for PF 5274857 hydrochloride

PF 5274857 hydrochloride is also offered as part of the Tocriscreen 2.0 Max. Find out more about compound libraries available from Tocris.

Technical Data for PF 5274857 hydrochloride

M. Wt	473.42
Formula	C₂₀H₂₅ClN₄O₃S.HCl
Storage	Desiccate at RT
Purity	≥98% (HPLC)
CAS Number	1613439-62-5
PubChem ID	90489027
InChi Key	GULNFUQAVPTTQV-UHFFFAOYSA-N
Smiles	CC1=CN=C(C2=CC(N3CCN(C(CCS(C)(=O)=O)=O)CC3)=NC=C2Cl)C(C)=C1.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for PF 5274857 hydrochloride

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	water	47.34	100
	DMSO	47.34	100

Preparing Stock Solutions for PF 5274857 hydrochloride

The following data is based on the product molecular weight 473.42.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.11 mL	10.56 mL	21.12 mL
5 mM	0.42 mL	2.11 mL	4.22 mL
10 mM	0.21 mL	1.06 mL	2.11 mL
50 mM	0.04 mL	0.21 mL	0.42 mL

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References for PF 5274857 hydrochloride

References are publications that support the biological activity of the product.

Rohner Effective targeting of Hedgehog signaling in a medulloblastoma model with PF-5274857, a potent and selective Smoothened antagonist that penetrates the blood-brain barrier. Mol.Cancer Ther. 2011 PMID: 22084163