Purvalanol B

Name: Purvalanol B
Brand: Tocris Bioscience
SKU: 1581

Tocris Bioscience, part of Bio-Techne | Catalog # 1581

Citations (20)

COA / SDS

Selective cdk inhibitor; potently inhibits cdk1, cdk2 and cdk5

Key Product Details

Description:	Selective cdk inhibitor; potently inhibits cdk1, cdk2 and cdk5
Alternative Names:	NG 95
Chemical Name:	(2R)-2-[[6-[(3-Chloro-4-carboxyphenyl)amino]-9-(1-methylethyl)-9H-purin-2-yl]amino]-3-methyl-1-butanol
Purity:	≥99% (HPLC)
Molecular Weight:	432.91

View Full Technical Details for Purvalanol B

Technical Data
Citations

Biological Activity for Purvalanol B

Purvalanol B is a cyclin-dependent kinase (cdk) inhibitor (reported IC₅₀ values are 6 nM for cdk1 and cdk5, and 6 - 9 nM for cdk2, depending on binding partner). Purvalanol B is selective over a range of other protein kinases (IC₅₀ >10,000 nM). Purvalanol B shows antiproliferative properties, mediated by ERK1 and ERK2. Purvalanol B induces autophagy in cellular models and induces apoptosis in cancer cells, the apoptotic effects can be increased by combining with Rapamycin (Cat. No. 1292).

Licensing Information

Sold under license from the Regents of the University of California

Compound Libraries for Purvalanol B

Purvalanol B is also offered as part of the Tocriscreen 2.0 Max and Tocriscreen Kinase Inhibitor Library. Find out more about compound libraries available from Tocris.

Technical Data for Purvalanol B

M. Wt	432.91
Formula	C₂₀H₂₅ClN₆O₃
Storage	Store at +4°C
Purity	≥99% (HPLC)
CAS Number	212844-54-7
PubChem ID	448991
InChi Key	ZKDXRFMOHZVXSG-HNNXBMFYSA-N
Smiles	CC(C)[C@H](CO)NC1=NC(NC2=CC(Cl)=C(C=C2)C(O)=O)=C2N=CN(C(C)C)C2=N1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Purvalanol B

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	1eq. NaOH	43.29	100 with gentle warming
	DMSO	43.29	100

Preparing Stock Solutions for Purvalanol B

The following data is based on the product molecular weight 432.91.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.31 mL	11.55 mL	23.10 mL
5 mM	0.46 mL	2.31 mL	4.62 mL
10 mM	0.23 mL	1.15 mL	2.31 mL
50 mM	0.05 mL	0.23 mL	0.46 mL

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References for Purvalanol B

References are publications that support the biological activity of the product.

Ozfiliz-Kilbas Cyclin-dependent kinase inhibitors, roscovitine and purvalanol, induce apoptosis and autophagy related to unfolded protein response in HeLa cervical cancer cells. Mol.Biol.Rep. 2018 PMID: 29978381
Jorda How selective are pharmacological inhibitors of cell-cycle-regulating cyclin-dependent kinases? J.Med.Chem. 2018 PMID: 30234987
Gray ATP-site directed inhibitors of cyclin-dependent kinases. Curr.Med.Chem. 1999 PMID: 10495356
Knockaert p42/p44 MAPKs are intracellular targets of the CDK inhibitor purvalanol. Oncogene 2002 PMID: 12226745
Gray Exploiting chemical libraries, structure, and genomics in the search for kinase inhibitors. Science 1998 PMID: 9677190