SAG dihydrochloride

Name: SAG dihydrochloride
Brand: Tocris Bioscience
SKU: 6390
Rating: 5 (1 reviews)

Tocris Bioscience, part of Bio-Techne | Catalog # 6390

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Citations (3)

COA / SDS

Dihydrochloride salt of SAG; Smo receptor agonist

Key Product Details

Description:	Dihydrochloride salt of SAG; Smo receptor agonist
Chemical Name:	3-Chloro-N-[trans-4-(methylamino)cyclohexyl]-N-[[3-(4-pyridinyl)phenyl]methyl]benzo[b]thiophene-2-carboxamide dihydrochloride
Purity:	≥98% (HPLC)
Molecular Weight:	562.98

View Full Technical Details for SAG dihydrochloride

Technical Data
Citations / Reviews (1)

Biological Activity for SAG dihydrochloride

SAG dihydrochloride is a water-soluble dihydrochloride salt of SAG (Cat. No. 4366). SAG is a potent Smoothened (Smo) receptor agonist (Kd = 59 nM); antagonizes Cyclopamine (Cat. No. 1623) action at the Smo receptor. SAG potently activates the Hedgehog signaling pathway in Shh-light 2 cells (EC₅₀ ~ 3 nM) and induces pathway activation independently of Ptch proteins. SAG is a putative inhibitor of a cellular component required for Hedgehog signaling and also enhances neuronal differentiation of iPSCs into dopaminergic neurons. The compound can be used in protocols for the chemical reprogramming of somatic cells to iPSCs.

Compound Libraries for SAG dihydrochloride

SAG dihydrochloride is also offered as part of the Tocriscreen 2.0 Max. Find out more about compound libraries available from Tocris.

Technical Data for SAG dihydrochloride

M. Wt	562.98
Formula	C₂₈H₂₈ClN₃OS.2HCl
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	2702366-44-5
PubChem ID	71433770
InChi Key	SWBYSYANHQTINY-CZBZHNTRSA-N
Smiles	ClC1=C(C(N([C@H]2CC[C@H](NC)CC2)CC3=CC=CC(C4=CC=NC=C4)=C3)=O)SC5=CC=CC=C51.Cl.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for SAG dihydrochloride

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	water	56.3	100
	DMSO	56.3	100

Preparing Stock Solutions for SAG dihydrochloride

The following data is based on the product molecular weight 562.98.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.78 mL	8.88 mL	17.76 mL
5 mM	0.36 mL	1.78 mL	3.55 mL
10 mM	0.18 mL	0.89 mL	1.78 mL
50 mM	0.04 mL	0.18 mL	0.36 mL

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References for SAG dihydrochloride

References are publications that support the biological activity of the product.

Guan Chemical reprogramming of human somatic cells to pluripotent stem cells. Nature 2022 PMID: 35418683