Assess Transcriptionally Active HPV Biomarkers in Head and Neck Cancer Biopsies

Evidence for transcriptional activity of the viral oncogenes E6/E7 is regarded as the gold standard for presence of clinically relevant high-risk human papillomavirus (HPV), but detection of E6/E7 mRNA can be challenging using conventional techniques. As a causal agent in head and neck squamous cell carcinoma (HNSCC), it is critical that the detection method for E6/E7 mRNA enables pathologist review of tissue morphology and is of the highest specificity and sensitivity for accurate assessment of the tissue microenvironment of FFPE specimens. RNAscope™ HPV Biomarker Detection Reagents and its proprietary “double Z” oligonucleotide probes specific for each subtype E6/E7 mRNA enable high specificity detection of viral transcripts in routine FFPE tumor biopsies.