Simple Western Assays
Simple Western™ is a fully automated capillary electrophoresis solution that seamlessly combines CE-SDS and cIEF with immunodetection using conventional antibodies. Simple Western makes your protein characterization a breeze!
Simple Western Assays Separate Proteins in Two Ways
- Simple Western Size. Separate proteins by molecular weight using CE-SDS plus immunodetection.
- Simple Western Charge. Separate proteins by isoelectric point (pI) using cIEF plus immunodetection.
Our Simple Western instruments offer Size, Charge, or both! No matter what option you choose, you'll get immunodetection of your target protein and truly quantitative data.
Benefits of Simple Western Size |
Benefits of Simple Western Charge |
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| Hands-Free Automation: Simple Western Size assays fully automate all assay steps, including sample loading, protein separation, immunoprobing, or total protein labeling, washing, detection, and data analysis. Just add your SDS-denatured samples and detection reagents onto the plate and press Start. | Hands-Free Automation: Simple Western Charge assays fully automate all assays steps, including sample loading, protein separation, immunoprobing, detection, and data analysis. Just mix your sample with our ampholytes sample mix, pipet onto your plate and get data for your target in its native state. |
| Reproducible Quantitation: Compared to traditional Western blotting, where data is semi-quantitative at best, Simple Western Size assays provide reliable absolute or relative quantitation of your target with low inter and intra-assay CVs. | Reproducible Quantitation: Compared to traditional gel-based IEF assays, where data is semi-quantitative at best and assay set up is more of an art than science, Simple Western Charge assays provide reliable absolute or relative quantitation of your target as well as unique isoform information, with low inter and intra-assay CVs. |
| High Throughput: Simple Western Size assays can process 25 samples in 3 hours or up to 96 samples overnight on our higher throughput platforms, giving you fully analyzed and quantified results. | High Throughput: Simple Western Charge assays can process up to 96 samples in one overnight run with reported pIs and quantitative results. |
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Low Sample Input: With minimum sample loading volumes of 3 µL per well, Simple Western Size assays can get you the data you need using as little of your precious sample as possible. Our high throughput systems can provide as many as 8 data points out of a 5 µL sample. |
Low Sample Input: Low abundance proteins in limited cell populations are our specialty, and as few as 25 cells per assay are required. Our high throughput systems can provide as many as 8 data points out of a 10 µL sample. |
| Open Platform: Transition is simple— most antibodies used for Western blotting protocols can be used with Simple Western Size assays. | Open Platform: Transition is simple—the same antibodies used for Western blotting protocols can be used with Simple Western Charge assays. |
| High Sensitivity Multiplex. Stellar™ NIR/IR Modules set the industry standard for Western blotting fluorescence detection sensitivity. Superplex across 2-color fluorescence and chemiluminescence channels with simultaneous total protein normalization. Perform sequential multiplex immunoassays or total protein detection with RePlex™ and reduce the cost per data point compared to Western blot. | Flexibility: Characterize a wide range of mechanisms, from kinase inhibitor drug to oncoprotein control and more, in a variety of sample type, including primary tissues and fine-needle aspirates. |