alpha Tubulin Antibody (DM1A) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB100-690
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Porcine, Avian, Bovine, Canine, Chicken, Chinese Hamster, Drosophila, Fungi, Goat, Guinea Pig, Hamster, Monkey, Parasite, Primate, Rabbit, Xenopus, Yeast
Cited:
Human, Mouse, Rat, Avian - Chicken, Canine, Frog - Xenopus (African Clawed Frog), Fungi, Goat, Hamster, Insect - Drosophila, Primate, Primate - Macaca mulatta (Rhesus Macaque), Rabbit, Yeast
Applications
Validated:
CyTOF-ready, Flow (Intracellular), Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunomicroscopy, Immunoprecipitation, Simple Western, Western Blot
Cited:
Block/Neutralize, Flow Cytometry, IF/IHC, Immunocytochemistry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # DM1A
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
This alpha Tubulin Antibody (DM1A) was developed against native chicken brain microtubules.
Reactivity Notes
Use in Mouse reported in scientific literature (PMID:34871568) Use in Mouse reported in scientific literature (PMID:34533563). Yeast reactivity reported in scientific literature (PMID: 25126732). Goat reactivity reported in scientific literature (PMID:31805146). Will likely react with all mammals.
Specificity
This alpha Tubulin Antibody (DM1A) does not cross-react with beta Tubulin.
Marker
Microtubule Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
As the TUBA1A gene is conserved evolutionarily and is ubiquitously expressed in most eukaryotic cell lines,
the Alpha tubulin antibody has been shown to be an attractive and effective choice for
a loading control, detecting at approximately 50-55 kDa. Quantitative western blotting requires a loading
control in order to account and adjust for the differences in the loading of samples across wells.
Scientific Data Images for alpha Tubulin Antibody (DM1A) - BSA Free
Simple Western: alpha Tubulin Antibody (DM1A) [NB100-690] - Simple Western lane view shows a specific band for alpha Tubulin in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Alpha tubulin molecular weight: 50 kDa.
Immunohistochemistry: alpha Tubulin Antibody (DM1A) [NB100-690] - Analysis of formalin fixed colon sections. Heat mediated antigen retrieval was performed using sodium citrate buffer for 20 min before incubating with primary antibody at a 0.5ug/ml dilution for 15 min at RT.
Immunohistochemistry: alpha Tubulin Antibody (DM1A) [NB100-690] - Analysis of colon tissue. Sections were formalin fixed and embedded with paraffin. Sodium citrate heat mediated antigen retrieval for 20 min. Incubated with primary antibody for 15 min at a 5 ug/ml concentration. Corner image is staining with secondary only.
Applications for alpha Tubulin Antibody (DM1A) - BSA Free
Application
Recommended Usage
Flow Cytometry
1 ug per million cells
Immunocytochemistry/Immunofluorescence
1:50000-1:100000
Immunohistochemistry
1:100-1:500
Immunohistochemistry-Frozen
1:100-1:500
Immunohistochemistry-Paraffin
1:100-1:500
Immunoprecipitation
1:50-1:100
Simple Western
1:50
Western Blot
1:5000
Application Notes
This alpha Tubulin Antibody (DM1A) is useful as a loading control for Western blot as well as Immunoprecipitation, Immunohistochemistry on paraffin-embedded and frozen sections, Immunocytochemistry/Immunofluorescence and Flow Cytometry.
The DM1A alpha tubulin antibody is ideal for use as a Western blot loading control, where a band can be seen around 50-55 kDa and as a cytoskeletal marker in ICC. For IHC-Paraffin, antigen retrieval is not essential, but may optimize staining.
Simple Western reported by an internal validation. Separated by Size-Jess/Wes, Sally Sue/Peggy Sue; matrix was 12-230 kDa. Only 10 - 15 ul of the recommended dilution is used per data point.
This antibody is CyTOF ready.
The DM1A alpha tubulin antibody is ideal for use as a Western blot loading control, where a band can be seen around 50-55 kDa and as a cytoskeletal marker in ICC. For IHC-Paraffin, antigen retrieval is not essential, but may optimize staining.
Simple Western reported by an internal validation. Separated by Size-Jess/Wes, Sally Sue/Peggy Sue; matrix was 12-230 kDa. Only 10 - 15 ul of the recommended dilution is used per data point.
This antibody is CyTOF ready.
Reviewed Applications
Read 14 reviews rated 4.6 using NB100-690 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
BSA Free
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: alpha Tubulin
Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation which is characteristic for microtubules in neuronal cells and the mitotic spindle.
Like GAPDH and beta-actin, alpha/beta tubulin is often used as a loading control in immunoblot applications (1). Alpha/beta tubulin is also good for counterstaining microtubules in immunofluorescence (2).
References
1. Hannen, R., Selmansberger, M., Hauswald, M., Pagenstecher, A., Nist, A., Stiewe, T., . . . Bartsch, J. W. (2019). Comparative Transcriptomic Analysis of Temozolomide Resistant Primary GBM Stem-Like Cells and Recurrent GBM Identifies Up-Regulation of the Carbonic Anhydrase CA2 Gene as Resistance Factor. Cancers (Basel), 11(7). doi:10.3390/cancers11070921
2. Nel, M., Joubert, A. M., Dohle, W., Potter, B. V., & Theron, A. E. (2018). Modes of cell death induced by tetrahydroisoquinoline-based analogs in MDA-MB-231 breast and A549 lung cancer cell lines. Drug Des Devel Ther, 12, 1881-1904. doi:10.2147/dddt.S152718
Long Name
Tubulin Alpha 1a
Alternate Names
Alpha-Tubulin 3, B-ALPHA-1, LIS3, TUBA1A, TUBA3, Tubulin B-Alpha-1, alpha tubulin loading control, alpha tubulin monoclonal, DM1A, DM1A anti-tubulin, dm1a monoclonal, DM1a tubulin, TUBA1 monoclonal, tubulin monoclonal
Entrez Gene IDs
7846 (Human)
Gene Symbol
TUBA1A
Additional alpha Tubulin Products
Product Documents for alpha Tubulin Antibody (DM1A) - BSA Free
Product Specific Notices for alpha Tubulin Antibody (DM1A) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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