alpha Tubulin Antibody (DM1A) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80570
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat, Porcine, Avian, Bovine, Canine, Chicken, Chinese Hamster, Drosophila, Fungi, Goat, Guinea Pig, Hamster, Monkey, Parasite, Primate, Rabbit, Xenopus, Yeast
Applications
CyTOF-ready, Flow (Intracellular), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunomicroscopy, Immunoprecipitation, Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # DM1A
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
This alpha Tubulin Antibody (DM1A) - Azide and BSA Free was developed against native chicken brain microtubules.
Reactivity Notes
Yeast reactivity reported in scientific literature (PMID: 25126732). Will likely react with all mammals.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
As the TUBA1A gene is conserved evolutionarily and is ubiquitously expressed in most eukaryotic cell lines,
the Alpha tubulin antibody has been shown to be an attractive and effective choice for
a loading control, detecting at approximately 50-55 kDa. Quantitative western blotting requires a loading
control in order to account and adjust for the differences in the loading of samples across wells.
Scientific Data Images for alpha Tubulin Antibody (DM1A) - Azide and BSA Free
Western Blot: alpha Tubulin Antibody (DM1A) - Azide and BSA Free [NBP2-80570] - Western blot analysis of extracts from HeLa, COS and C6 cells using alpha Tubulin antibody (NB100-690, 1:1000, Alpha tubulin molecular weight: 50 kDa) Image from the standard format of this antibody.
Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (DM1A) - Azide and BSA Free [NBP2-80570] - Analysis of HeLa cells, green staining is alpha tubulin whereas red is DNA stained with propidium iodide. Image from the standard format of this antibody.
Immunohistochemistry: alpha Tubulin Antibody (DM1A) - Azide and BSA Free [NBP2-80570] - Staining of the marine parasite Cryptocaryon irritans mouth. Large bundles of microtubules form a cytophyrigeal basket. Image from the standard format of this antibody.
Applications for alpha Tubulin Antibody (DM1A) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1 ug per million cells
Immunocytochemistry/ Immunofluorescence
1:50000-1:100000
Immunohistochemistry-Frozen
1:100-1:500
Immunoprecipitation
1:50-1:100
Simple Western
1:50
Western Blot
1:5000
Application Notes
This alpha Tubulin Antibody (DM1A) is useful as a loading control for Western blot as well as Immunoprecipitation, Immunohistochemistry on paraffin-embedded and frozen sections, Immunocytochemistry/Immunofluorescence and Flow Cytometry.
The DM1A alpha tubulin antibody is ideal for use as a Western blot loading control, where a band can be seen around 50-55 kDa and as a cytoskeletal marker in ICC. For IHC-Paraffin, antigen retrieval is not essential, but may optimize staining.
This antibody is CyTOF ready.
The DM1A alpha tubulin antibody is ideal for use as a Western blot loading control, where a band can be seen around 50-55 kDa and as a cytoskeletal marker in ICC. For IHC-Paraffin, antigen retrieval is not essential, but may optimize staining.
This antibody is CyTOF ready.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: alpha Tubulin
Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation which is characteristic for microtubules in neuronal cells and the mitotic spindle.
Like GAPDH and beta-actin, alpha/beta tubulin is often used as a loading control in immunoblot applications (1). Alpha/beta tubulin is also good for counterstaining microtubules in immunofluorescence (2).
References
1. Hannen, R., Selmansberger, M., Hauswald, M., Pagenstecher, A., Nist, A., Stiewe, T., . . . Bartsch, J. W. (2019). Comparative Transcriptomic Analysis of Temozolomide Resistant Primary GBM Stem-Like Cells and Recurrent GBM Identifies Up-Regulation of the Carbonic Anhydrase CA2 Gene as Resistance Factor. Cancers (Basel), 11(7). doi:10.3390/cancers11070921
2. Nel, M., Joubert, A. M., Dohle, W., Potter, B. V., & Theron, A. E. (2018). Modes of cell death induced by tetrahydroisoquinoline-based analogs in MDA-MB-231 breast and A549 lung cancer cell lines. Drug Des Devel Ther, 12, 1881-1904. doi:10.2147/dddt.S152718
Long Name
Tubulin Alpha 1a
Alternate Names
Alpha-Tubulin 3, B-ALPHA-1, LIS3, TUBA1A, TUBA3, Tubulin B-Alpha-1
Gene Symbol
TUBA1A
Additional alpha Tubulin Products
Product Documents for alpha Tubulin Antibody (DM1A) - Azide and BSA Free
Product Specific Notices for alpha Tubulin Antibody (DM1A) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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