beta-Catenin [p Ser552] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-05441

Novus Biologicals, part of Bio-Techne
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NBP3-05441
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all beta-Catenin Antibodies »

Product Summary for beta-Catenin [p Ser552] Antibody

Immunogen

A synthetic phosphorylated peptide around S552 of human beta-Catenin (NP_001091679).

Modification

p Ser552

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for beta-Catenin [p Ser552] Antibody

Western Blot: beta-Catenin [p Ser552] Antibody [NBP3-05441] - Western blot analysis of extracts of C2C12 cells, using beta-Catenin pAb (NBP3-05441) at 1:1000 dilution or beta-Catenin antibody (A11932).C2C12 cell lysates were treated by CIP at 37C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit. Exposure time: 30s.
Western Blot: beta-Catenin [p Ser552] Antibody [NBP3-05441] - Western blot analysis of extracts of various cell lines, using beta-Catenin [p Ser552] antibody (NBP3-05441) at 1:1000 dilution. C2C12 cell lysates were treated by CIP at 37C for 1 hour. NIH/3T3 cells were treated by Forskolin (30uM) for 30 minutes after serum-starvation overnight or treated by CIP at 37C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit. Exposure time: 30s.

Applications for beta-Catenin [p Ser552] Antibody

Application
Recommended Usage

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS with 50% glycerol, pH7.3.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: beta-Catenin

The protein encoded by the beta Catenin gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs arenecessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion betweencells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contactinhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this proteinbinds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this geneare a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Three transcript variants encoding the same protein have been found for this gene.

Alternate Names

bCatenin, CTNNB1

Gene Symbol

CTNNB1

Additional beta-Catenin Products

Product Documents for beta-Catenin [p Ser552] Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for beta-Catenin [p Ser552] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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