Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Bovine (91%), Canine (92%), Primate (99%), Rat (90%). Backed by our 100% Guarantee.

Applications

Flow (Intracellular), Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 1C2.2D11

Format

BSA Free

Concentration

1.0 mg/ml

View all Calnexin Antibodies »

Product Specifications

Immunogen

Partial recombinant human Calnexin protein (between amino acids 1-300). [UniProt P27824]

Reactivity Notes

Immunogen's sequence similarity with other species: Mouse (88%), Duck (81%), Xenopus (76%)

Localization

Endoplasmic reticulum membrane, Melanosome

Marker

Endoplasmic Reticulum Membrane Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for Calnexin Antibody (1C2.2D11) - BSA Free

Western Blot: Calnexin Antibody (1C2.2D11)BSA Free [NBP2-36570]

Western Blot: Calnexin Antibody (1C2.2D11)BSA Free [NBP2-36570]

Western Blot: Calnexin Antibody (1C2.2D11) [NBP2-36570] - Analysis of 11 kDa Partial Recombinant Human Calnexin protein with Calnexin antibody (clone 1C2.2D11) at 0.5 ug/mL.
Immunocytochemistry/ Immunofluorescence: Calnexin Antibody (1C2.2D11) - BSA Free [NBP2-36570]

Immunocytochemistry/ Immunofluorescence: Calnexin Antibody (1C2.2D11) - BSA Free [NBP2-36570]

Immunocytochemistry/Immunofluorescence: Calnexin Antibody (1C2.2D11) [NBP2-36570] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-Calnexin (1C2.2D11) conjugated to FITC [NBP2-36570F] at 10ug/mL for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: Calnexin Antibody (1C2.2D11) - BSA Free [NBP2-36570]

Immunohistochemistry-Paraffin: Calnexin Antibody (1C2.2D11) - BSA Free [NBP2-36570]

Immunohistochemistry-Paraffin: Calnexin Antibody (1C2.2D11) [NBP2-36570] - Analysis of FFPE tissue section of normal human kidney using mouse monoclonal Calnexin antibody (clone 1C2.2D11) at 7 ug/mL concentration. The cells of Glomeruli developed strong cytoplasmic staining.
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Applications for Calnexin Antibody (1C2.2D11) - BSA Free

Application
Recommended Usage

Flow (Intracellular)

5 ug/million cells

Immunocytochemistry/Immunofluorescence

10 ug/ml

Immunohistochemistry

7 ug/ml

Immunohistochemistry-Paraffin

7 ug/ml

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Calnexin

Calnexin is a calcium-binding protein which interacts with newly synthesized glycoproteins in ER for facilitating protein assembly and/or the retention within ER of unassembled protein subunits as well as incorrectly folded proteins thereby playing a key role in ER's quality control apparatus. The specificity of calnexin for glycoproteins is defined by a lectin site, which binds an early oligosaccharide intermediate on the folding glycoprotein. When associated with partial T-cell antigen receptor complexes which escape immature thymocytes's ER, calnexin regulates thymocyte maturation process and is also implicated in receptor-mediated endocytosis at synapses. MAPK3/ERK1 phosphorylates calnexin at Ser-565 leading to increased calnexin-ribosomal association and DHHC6 mediated calnexin palmitoylation leads to its preferential localization to perinuclear rough ER. Palmitoylation also facilitate calnexin association with RTC/ribosome-translocon complex that is essential to efficient folding of glycosylated proteins.

Alternate Names

calnexin, CNX, IP90FLJ26570, Major histocompatibility complex class I antigen-binding protein p88, P90

Entrez Gene IDs

821 (Human); 12330 (Mouse); 29144 (Rat)

Gene Symbol

CANX

UniProt

P27824 (Human)

Additional Calnexin Products

Product Documents for Calnexin Antibody (1C2.2D11) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Calnexin Antibody (1C2.2D11) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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