Key Product Details

Species Reactivity

Validated:

Human, Rat (Negative)

Cited:

Human

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

WB

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # CANX/1541

Concentration

0.2 mg/ml

View all Calnexin Antibodies »

Product Summary for Calnexin Antibody (CANX/1541)

Immunogen

Recombinant N-terminal fragment of human Calnexin protein (around aa 1-300) (exact sequence is proprietary) (Uniprot: P27824)

Reactivity Notes

Does not react with Rat.

Localization

Cytoplasmic

Marker

Endoplasmic Reticulum Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Theoretical MW

90 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-54552)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Calnexin Antibody (CANX/1541)

Western Blot: Calnexin Antibody (CANX/1541) [NBP2-53352] - Western Blot Analysis of HeLa and MCF-7 cell lysate using Calnexin Antibody (CANX/1541).
Immunocytochemistry/Immunofluorescence: Calnexin Antibody (CANX/1541) [NBP2-53352] - Confocal immunofluorescence image of HeLa cells using Calnexin Antibody (CANX/1541), labeled in Green. Red Dot is used to label the nuclei Red.
Immunohistochemistry-Paraffin: Calnexin Antibody (CANX/1541) [NBP2-53352] - Formalin-fixed, paraffin-embedded human Small Intestinal Carcinoma stained with Calnexin Monoclonal Antibody (CANX/1541).
View 3 more images

Applications for Calnexin Antibody (CANX/1541)

Application
Recommended Usage

ELISA

2-4 ug/ml

Immunocytochemistry/ Immunofluorescence

0.5 - 1 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
ELISA: Use Ab at 2-4ug/ml for coating. Order Ab without BSA.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Calnexin

Calnexin, an abundant approx. 90kDa integral protein of the endoplasmic reticulum, is also referred to as IP90, p88 and p90 (1). It consists of a large 50kDa N-terminal calcium-binding luminal domain, a single transmembrane helix and a short acidic cytoplasmic tail (2, 3). Unlike its ER counterparts which have a KDEL sequence on their Cterminus to ensure ER retention (4), calnexin has positively charged cytosolic residues that do the same thing (3). Most ER proteins act as molecular chaperones and participate in the proper folding of polypeptides and their assembly into mulit-subunit proteins. Calnexin together with calreticulin, plays a key role in glycoprotein folding and its control within the ER, by interacting with folding intermediates via their monoglycosylated glycans (5, 6). Calnexin has also been shown to associate with the major histocompatability complex class I heavy chains, partial complexes of the T cell receptor and B cell membrane immunoglobulin (7).

Alternate Names

calnexin, CNX, IP90FLJ26570, Major histocompatibility complex class I antigen-binding protein p88, p90

Gene Symbol

CANX

UniProt

P27824 (Human)

Additional Calnexin Products

Product Documents for Calnexin Antibody (CANX/1541)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Calnexin Antibody (CANX/1541)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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