Calnexin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-97476

Novus Biologicals, part of Bio-Techne
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NBP1-97476-0.025mg
NBP1-97476-0.05mg
NBP1-97476-0.2mg
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Bovine, C. elegans, Canine, Chicken, Guinea Pig, Hamster, Primate, Rabbit, Sheep, Xenopus

Cited:

Human, C. elegans

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Single Cell Western, Western Blot

Cited:

WB

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Calnexin Antibodies »

Product Summary for Calnexin Antibody

Immunogen

Synthetic peptide corresponding to the sequence near the N-terminus of canine calnexin.

Marker

Endoplasmic Reticulum Membrane Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

90 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Calnexin Antibody

Western Blot: Calnexin Antibody [NBP1-97476] - Lane 1: MWM, Lane 2: Vero, Lane 3: 3T3, Lane 4: PC-12, Lane 5: HeLa.
Immunohistochemistry-Paraffin: Calnexin Antibody [NBP1-97476] - Analysis of human placenta tissue stained with Calnexin, pAb at 5ug/ml.

Applications for Calnexin Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:10-1:500

Single Cell Western

1:10

Western Blot

1:1000
Application Notes
Single Cell Western reported by an internal validation on U373 MG cells at a 1:10 dilution
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Calnexin

Calnexin (CNX), an unglycosylated resident ER transmembrane protein, together with Calreticulin (CRT), plays a key role in glycoprotein folding and its control within the ER, by interacting with folding intermediates via their monoglucosylated glycans. Calnexin associates with newly synthesized monomeric glycoproteins and only recognizes glycoproteins when they are incompletely folded. Furthermore, Calnexin has been demonstrated to function as a molecular chaperone capable of interacting with polypeptide segments of folding glycoproteins.

Alternate Names

calnexin, CNX, IP90FLJ26570, Major histocompatibility complex class I antigen-binding protein p88, p90

Gene Symbol

CANX

Additional Calnexin Products

Product Documents for Calnexin Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Calnexin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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