Human APRIL/TNFSF13 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF884
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
CyTOF-ready, ELISA, Flow Cytometry, Western Blot
Cited:
ELISA Development, Flow Cytometry, Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Summary for Human APRIL/TNFSF13 Antibody
Specificity
Detects human APRIL/TNFSF13 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human (rh) Fas Ligand, rhTNF-alpha, rhTRAIL, and rhTRANCE is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human APRIL/TNFSF13 Antibody
Human APRIL/TNFSF13 ELISA Standard Curve.
Recombinant Human APRIL/TNFSF13 protein was serially diluted 2-fold and captured by Mouse Anti-Human APRIL/TNFSF13 Monoclonal Antibody (MAB8844) coated on a Clear Polystyrene Microplate (DY990). Goat Anti-Human APRIL/TNFSF13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF884) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).Detection of APRIL/TNFSF13 in U937 Human Cell Line by Flow Cytometry.
U937 human lymphoma cell line was stained with Goat Anti-Human APRIL/TNFSF13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF884, filled histogram) or control antibody staining (AB-108-C, open histogram) followed by anti-Goat IgG PE-conjugated Secondary Antibody (F0107). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). View our protocol for Staining Intracellular Molecules.Applications for Human APRIL/TNFSF13 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
ELISA
This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human APRIL/TNFSF13 Monoclonal Antibody (Catalog # MAB8844).
This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human APRIL/TNFSF13 DuoSet ELISA Kit (Catalog # DY884B) for convenient development of a sandwich ELISA.
Flow Cytometry
0.25 µg/106 cells
Sample: U937 human lymphoma cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Sample: U937 human lymphoma cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Western Blot
0.1 µg/mL
Sample: Recombinant Human APRIL/TNFSF13 (Catalog # 884-AP)
Sample: Recombinant Human APRIL/TNFSF13 (Catalog # 884-AP)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Background: APRIL/TNFSF13
References
- Planelles, L. et al. (2008) Curr. Mol. Med. 8:829.
- Hahne, M. et al. (1998) J. Exp. Med. 188:1185.
- Lopez-Fraga, M. et al. (2001) EMBO Reports 2:945.
- Kelly, K. et al. (2000) Cancer Res. 60:1021.
- Roth, W. et al. (2001) Cell Death Differ. 8:403.
- Yu, G. et al. (2000) Nat. Immunol. 1:252.
- He, B. et al. (2004) J. Immunol. 172:3268.
- Schwaller, J. et al. (2007) Blood 109:331.
- Mhawech-Fauceglia, P. et al. (2008) Eur. J. Cancer 44:2097.
- Day, E.S. et al. (2005) Biochemistry 44:1919.
- Bossen, C. et al. (2006) J. Biol. Chem. 281:13964.
- Hendriks, J. et al. (2005) Cell Death Differ. 12:637.
- Ingold, K. et al. (2005) J. Exp. Med. 201:1375.
- Roschke, V. et al. (2002) J. Immunol. 169:4314.
- Pradet-Balade, B. et al. (2002) EMBO J. 21:5711.
Long Name
A Proliferation-inducing Ligand
Alternate Names
CD256, TALL2, TNFSF13, TRDL1, ZTNF2
Gene Symbol
TNFSF13
Additional APRIL/TNFSF13 Products
Product Documents for Human APRIL/TNFSF13 Antibody
Product Specific Notices for Human APRIL/TNFSF13 Antibody
For research use only
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