Human APRIL/TNFSF13 Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAF884

R&D Systems, part of Bio-Techne
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BAF884
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Western Blot

Cited:

ELISA Development, Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG

View all APRIL/TNFSF13 Antibodies »

Product Specifications

Specificity

Detects human APRIL in Western blots. In this format, less than 1% cross-reactivity is observed with rhTRANCE, rhTNF-alpha, rhFas Ligand, rhTRAIL, rhGITR, and rhVEGI.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human APRIL/TNFSF13 Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human APRIL/TNFSF13 (Catalog # 884-AP)

Formulation, Preparation, and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: APRIL/TNFSF13

APRIL (a proliferation-inducing ligand), also known as TNFSF13, TALL2, and TRDL1, is a member of the TNF ligand superfamily (1). APRIL is synthesized as a 32 kDa type II transmembrane protein which is cleaved by furin in the Golgi to release a 17 kDa soluble molecule (2, 3). Secreted APRIL consists almost entirely of a single TNF homology domain (2, 3). Little or no transmembrane APRIL is expressed on the cell surface (3). Alternate splicing generates isoforms with short deletions at the N- or C-terminus (4). Human APRIL shares 85% aa sequence identity with mouse and rat APRIL. Among TNF superfamily ligands, BAFF shows the greatest sequence homology with APRIL, and the two proteins exhibit overlapping biological activities. APRIL promotes cellular proliferation and protects from apoptosis in normal and transformed cells (3, 5‑7). It is present in elevated amounts in a wide variety of cancers primarily due to expression by tumor-infiltrating neutrophils (4, 5, 7‑9). Both APRIL and BAFF bind and signal through the TNF superfamily receptors TACI and BCMA, and BAFF additionally functions through BAFF R (6, 10, 11). A stretch of basic amino acids at the N-terminus of APRIL is required for its interaction with heparan sulfate proteoglycans (HSPGs) (12, 13). Binding to HSPGs is independent of APRIL’s binding to TACI and BCMA (12, 13). Interaction with HSPGs serves to concentrate APRIL on the surface of cells, thereby favoring TACI- or BCMA-mediated effects (8, 9, 13). APRIL can form bioactive heterotrimers with BAFF, and these circulate in the serum of patients with rheumatic immune disorders (14). A bioactive protein known as TWE-PRIL consists of the intracellular domain, transmembrane segment, and stalk region of TWEAK fused to the TNF homology domain of APRIL (15). TWE-PRIL is expressed in monocytes and activated T cells, and in contrast to APRIL, it is presented on the cell surface (15).

References

  1. Planelles, L. et al. (2008) Curr. Mol. Med. 8:829.
  2. Hahne, M. et al. (1998) J. Exp. Med. 188:1185.
  3. Lopez-Fraga, M. et al. (2001) EMBO Reports 2:945.
  4. Kelly, K. et al. (2000) Cancer Res. 60:1021.
  5. Roth, W. et al. (2001) Cell Death Differ. 8:403.
  6. Yu, G. et al. (2000) Nat. Immunol. 1:252.
  7. He, B. et al. (2004) J. Immunol. 172:3268.
  8. Schwaller, J. et al. (2007) Blood 109:331.
  9. Mhawech-Fauceglia, P. et al. (2008) Eur. J. Cancer 44:2097.
  10. Day, E.S. et al. (2005) Biochemistry 44:1919.
  11. Bossen, C. et al. (2006) J. Biol. Chem. 281:13964.
  12. Hendriks, J. et al. (2005) Cell Death Differ. 12:637.
  13. Ingold, K. et al. (2005) J. Exp. Med. 201:1375.
  14. Roschke, V. et al. (2002) J. Immunol. 169:4314.
  15. Pradet-Balade, B. et al. (2002) EMBO J. 21:5711.

Long Name

A Proliferation-inducing Ligand

Alternate Names

CD256, TALL2, TNFSF13, TRDL1, ZTNF2

Entrez Gene IDs

8741 (Human); 69583 (Mouse); 287437 (Rat)

Gene Symbol

TNFSF13

Additional APRIL/TNFSF13 Products

Product Documents for Human APRIL/TNFSF13 Biotinylated Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Human APRIL/TNFSF13 Biotinylated Antibody

For research use only

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