Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Rat, Rabbit

Applications

Validated:

CyTOF-ready, Flow Cytometry, Immunohistochemistry, Neutralization

Cited:

Binding Assay, ELISA Detection, Flow Cytometry, Functional Assay, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Neutralization, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 44716

View all CXCR4 Antibodies »

Product Specifications

Specificity

Detects human CXCR4. It will also react with cells expressing feline CXCR4 but not rat CXCR4.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human CXCR4 Antibody

Detection of CXCR4 antibody in Jurkat Human Cell Line antibody by Flow Cytometry.

Detection of CXCR4 in Jurkat Human Cell Line by Flow Cytometry.

Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human CXCR4 Monoclonal Antibody (Catalog # MAB172, filled histogram) or isotype control antibody (Catalog # MAB004, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.
CXCR4 antibody in Human Lymph Node by Immunohistochemistry (IHC-P).

CXCR4 in Human Lymph Node.

CXCR4 was detected in immersion fixed paraffin-embedded sections of human lymph node using 15 µg/mL Human CXCR4 Monoclonal Antibody (Catalog # MAB172) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS003) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
CXCR4 antibody in Human Spleen by Immunohistochemistry (IHC-P).

CXCR4 in Human Spleen.

CXCR4 was detected in immersion fixed paraffin-embedded sections of human spleen using Mouse Anti-Human CXCR4 Monoclonal Antibody (Catalog # MAB172) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Applications for Human CXCR4 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Jurkat human acute T cell leukemia cell line

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human lymph node and human spleen

Neutralization

Measured by its ability to neutralize CXCL12/SDF-1 alpha-induced chemotaxis in the BaF3 mouse pro-B cell line transfected with human CXCR4. The Neutralization Dose (ND50) is typically 2.5-12 µg/mL in the presence of 1 ng/mL Recombinant Human/Feline/Rhesus Macaque CXCL12/SDF-1 alpha.

Reviewed Applications

Read 6 reviews rated 5 using MAB172 in the following applications:

Formulation, Preparation, and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: CXCR4

CXCR4, also known as CD184, is a G-protein-linked seven transmembrane spanning receptor that binds stromal cell-derived factor-1 (SDF-1). CXCR4 acts as a co-factor for T-cell tropic HIV-1 and -2 viral entry into cells. While primarily a membrane protein, CXCR4 undergoes trafficking and internalization in response to stimulation with phorbol esters and ligand (1). Cytoplasmic and nuclear localization of CXCR4 has been observed in colorectal and renal carcinomas (2,3) and it has been used as the basis of prognosis and metastatic state (3,4,5).

References

  1. Orsini, M.J. et al. (1999) J. Biol. Chem. 274:31076.
  2. Zagzag, D. et al. (2005) Cancer Res. 65:6178.
  3. Speetjens, F.M. et al. (2009) Cancer Microenvironment 2:1.
  4. Wang, L. et al. (2009) Oncology Reports 22:1333.
  5. Amara, S. et al. (2015) Cancer Biomark. 15:869.

Long Name

C-X-C Motif Chemokine Receptor 4

Alternate Names

CD184, D2S201E, FB22, Fusin, HM89, HSY3RR, LAP-3, LAP3, LCR1, LESTR, NPY3R, NPYRL, NPYY3R, WHIMS

Entrez Gene IDs

7852 (Human); 12767 (Mouse); 60628 (Rat); 483900 (Canine); 493676 (Feline)

Gene Symbol

CXCR4

Additional CXCR4 Products

Product Documents for Human CXCR4 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CXCR4 Antibody

For research use only

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