Human ICAM-1/CD54 Fluorescein-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # BBA20

R&D Systems, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Fluorescein (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # BBIG-I1 (11C81)

View all ICAM-1/CD54 Antibodies »

Product Summary for Human ICAM-1/CD54 Fluorescein-conjugated Antibody

Specificity

Stains human ICAM-1/CD54 on human ICAM-1 transfected COS cells. It does not bind COS cells transfected with E-Selectin, VCAM-1, or PECAM-1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human ICAM-1/CD54 Fluorescein-conjugated Antibody

Detection of ICAM-1/CD54 antibody in Human PBMCs antibody by Flow Cytometry.

Detection of ICAM‑1/CD54 in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD14 APC-conjugated Monoclonal Antibody (Catalog # FAB3832A) and either (A) Mouse Anti-Human ICAM-1/CD54 Fluorescein-conjugated Monoclonal Antibody (Catalog # BBA20) or (B) Mouse IgG1Fluorescein Isotype Control (Catalog # IC002F). View our protocol for Staining Membrane-associated Proteins.

Applications for Human ICAM-1/CD54 Fluorescein-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Human peripheral blood mononuclear cells (PBMCs)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 3 reviews rated 4.7 using BBA20 in the following applications:

Formulation, Preparation, and Storage

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: ICAM-1/CD54

ICAM-1 is a member of the immunoglobulin superfamily whose expression is up‑regulated on leukocytes, epithelial cells and resting endothelial cells in response to inflammatory signals. ICAM-1 binds the leukocyte integrins LFA-1 and Mac-1.

Long Name

Intercellular Adhesion Molecule 1

Alternate Names

CD54, ICAM1

Entrez Gene IDs

3383 (Human); 15894 (Mouse); 25464 (Rat)

Gene Symbol

ICAM1

Additional ICAM-1/CD54 Products

Product Documents for Human ICAM-1/CD54 Fluorescein-conjugated Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human ICAM-1/CD54 Fluorescein-conjugated Antibody

For research use only

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