Human/Mouse/Rat Olig2 Antibody Best Seller

Olig2 in Human Brain.

Olig2 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Goat Anti-Human/Mouse/Rat Olig2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2418) at 10 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Olig2 in Rat Midbrain.

Olig2 was detected in immersion fixed paraffin-embedded sections of rat midbrain using Goat Anti-Human/Mouse/Rat Olig2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2418) at 10 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of Mouse Olig2 by Immunocytochemistry/Immunofluorescence

Pathological analysis of l2hgdh-/- mice.The figure compares the histological and ultrastructural appearance of the brain of l2hgdh+/+ (left panels: A, B, E, G, H, K, L) and l2hgdh-/- mice (right panels: C, D, F, I, J, M, N). Hematoxylin and eosin staining shows low-magnification of the lateral part of the brain (A, C) at the level of the lateral ventricle (LV), the caudate putamen (striatum; CPu) and the anterior commissure, anterior part (aca). Higher magnification of pencils of Wilson are shown in panels B and D. Notice the satellite oligodendrocyte at B (arrow). Luxol fast blue staining of myelin is shown in E and F, highlighting the predominant presence of vacuoles in the pencils of Wilson of the striatum of the l2hgdh-/- mouse. Panels G-J are microphotographs from the junction between cortex and white matter in the vicinity of corpus callosum. Immunostaining of NeuN (G and I) shows that most vacuoles in the l2hgdh-/- mice are at distance from the nucleus of neurons (notice the abundance of vacuoles in the lower part of panel I, devoid of neurons) although a few vacuoles indent the nucleus of some neurons (arrowheads). However, the nucleus of satellite oligodendrocytes is also present in close contact to the vacuole indenting the nucleus of some neurons (arrows), preventing to identify clearly which cell type actually contains the peri-neuronal vacuole. In contrast, immunostaining of Olig2 (H and J) clearly shows that most vacuoles are in close contact to the nucleus of oligodendrocytes in the l2hgdh-/- mouse brain. Notice the small size of the nucleus of oligodendrocytes compared to the nucleus of neurons. Panels K-N are Transmission Electron Microscopy photographs of striatum tissue. Ultrastructural analysis (K-N) shows many empty-looking cell processes (asterisks in panels M and N), containing dilated cytoplasmic organelles, in the glia of l2hgdh-/- mice. The myelin sheath of many axons appears focally altered or disrupted (arrows at M and N), sometimes at the vicinity of empty-looking spaces. C, capillary lumen. The length of the scale bar is indicated in each panel. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25763823), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Rat Olig2 by Immunocytochemistry/Immunofluorescence

alpha-Syn PFF exposure to immature OLGs during differentiation-induced abnormal oligodendrogenesis. a Confocal images of oligodendroglial cells on day 4 after differentiation induction showing the colocalization of alpha-syn immunoreactivity and cleaved caspase-9 expression. The cells were incubated with 1 μM alpha-syn PFFs for 24 h (day 3–4) before fixation. White arrowheads indicate intracellular inclusions. The regions marked by dotted squares are highlighted in the magnified views. Scale bar = 5 μm. b Scheme showing the experimental protocols of 3 μM alpha-syn PFF exposure to oligodendroglial cells during two different phases (day 0–1, or day 3–4) of differentiation. c Cell viability analysis (WST assay) of day 8 OLGs, which were obtained through different protocols of alpha-syn PFF exposure during differentiation (no exposure, exposure on day 0–1, or exposure on days 3–4). N = 5, respectively, independent culture, Kruskal–Wallis, p*  0.05. d Immunoblot analysis of day 8 OLGs obtained via different protocols of alpha-syn PFF exposure during differentiation (no exposure, exposure on day 0–1, or exposure on days 3–4), showing the protein expression levels of oligodendroglial cell markers. e, f Quantification of the relative protein expression levels of MBP (e) and BCAS1 (f) in day 8 OLGs obtained using the above-mentioned protocols. The differences between the no exposure group and day 0–1 group in (E) (p = 0.0636) and in (F) (p = 0.1980) were not statistically significant. N = 5, respectively, independent culture, Kruskal–Wallis, p*  0.05, p**  0.01. g Graphical representation of in vitro analysis showing two patterns of alpha-syn PFF-induced maturation inhibition. alpha-Syn PFF exposure to differentiating OLGs during the BCAS1(+) cell-dominant phase resulted in decreased cell viability (left), while exposure during the OPC-dominant phase resulted in abnormal protein expression levels of OLG markers (right) Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32727582), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse Olig2 by Immunocytochemistry/Immunofluorescence

Early onset of oligodendrocyte differentiation in Ptprz-deficient mice.A, Immunohistochemistry of cultured oligodendrocyte precursor cells (OPCs, NG2-postive cells, red) and oligodendrocytes (OLs, MBP-postive cells, green) from Ptprz-deficient mice and wild-type mice. Scale bars, 100 µm. The percentages of OPCs and OLs among total cells (DAPI-positive nuclei, blue) are shown at the right of each panel. Data are the mean ± SEM from five independent experiments. *p0.05 and **p0.01 (Student's t-test). B, Morphological assessment of cultured OLs at DIV6. The MBP-postive cells were classified into four categories. Representative images are shown in lower panels; Stage 1, three or less primary processes longer than a cell body with minimal development of secondary and tertiary processes; Stage 2, three or more primary processess with moderate secondary and tertiary processes; Stage 3, five or more primary processes with extensive secondry and teriary processes; Stage 4, extending myelin-like membrane structures and branched processes. Scale bars, 50 µm. Data are the mean ± SEM from three independent experiments. *p0.05 (Mann-Whitney U-test). C, Ptprz expression in oligodendrocyte lineage cells. Cultured cells at DIV10 were triple stained with anti-RPTP beta (specific for Ptprz receptor isoforms, green), anti-Olig2 (red), and DAPI (blue). Scale bars, 50 µm. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0048797), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse Olig2 by Immunocytochemistry/Immunofluorescence

Pathological analysis of l2hgdh-/- mice.The figure compares the histological and ultrastructural appearance of the brain of l2hgdh+/+ (left panels: A, B, E, G, H, K, L) and l2hgdh-/- mice (right panels: C, D, F, I, J, M, N). Hematoxylin and eosin staining shows low-magnification of the lateral part of the brain (A, C) at the level of the lateral ventricle (LV), the caudate putamen (striatum; CPu) and the anterior commissure, anterior part (aca). Higher magnification of pencils of Wilson are shown in panels B and D. Notice the satellite oligodendrocyte at B (arrow). Luxol fast blue staining of myelin is shown in E and F, highlighting the predominant presence of vacuoles in the pencils of Wilson of the striatum of the l2hgdh-/- mouse. Panels G-J are microphotographs from the junction between cortex and white matter in the vicinity of corpus callosum. Immunostaining of NeuN (G and I) shows that most vacuoles in the l2hgdh-/- mice are at distance from the nucleus of neurons (notice the abundance of vacuoles in the lower part of panel I, devoid of neurons) although a few vacuoles indent the nucleus of some neurons (arrowheads). However, the nucleus of satellite oligodendrocytes is also present in close contact to the vacuole indenting the nucleus of some neurons (arrows), preventing to identify clearly which cell type actually contains the peri-neuronal vacuole. In contrast, immunostaining of Olig2 (H and J) clearly shows that most vacuoles are in close contact to the nucleus of oligodendrocytes in the l2hgdh-/- mouse brain. Notice the small size of the nucleus of oligodendrocytes compared to the nucleus of neurons. Panels K-N are Transmission Electron Microscopy photographs of striatum tissue. Ultrastructural analysis (K-N) shows many empty-looking cell processes (asterisks in panels M and N), containing dilated cytoplasmic organelles, in the glia of l2hgdh-/- mice. The myelin sheath of many axons appears focally altered or disrupted (arrows at M and N), sometimes at the vicinity of empty-looking spaces. C, capillary lumen. The length of the scale bar is indicated in each panel. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25763823), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Olig2 in Human Cortex.

Formalin-fixed paraffin-embedded tissue sections of human cerebral cortex were probed for Olig2 mRNA (ACD RNAScope Probe, catalog #424198; Fast Red chromogen, ACD catalog # 322750). Adjacent tissue section was processed for immunohistochemistry using goat anti-human Olig2 polyclonal antibody (R&D Systems catalog # AF2418) at 3ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-goat IgG VisUCyte HRP Polymer Antibody (Catalog # VC004) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to glial cells.