ICAM-1/CD54 Antibody (1A29)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-22541
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse, Rat
Applications
Validated:
Block/Neutralize, Electron Microscopy, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry Free-Floating, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Proximity Ligation Assay, Western Blot
Cited:
B/N, EM, FLOW, IF/IHC, IHC-FrFl, IHC-P, PLA, Simple Western, WB
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 1A29
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Rat lymph node stroma
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for ICAM-1/CD54 Antibody (1A29)
Western Blot: ICAM-1/CD54 Antibody (1A29) [NBP2-22541] - Western blot analysis of MDA-MB-231 cells transfected with/without an IL-8 silencer RNA (siIL-8) to evaluate the expression of MUC-1, VCAM-1 (NBP1-28404), and ICAM-1 (NBP2-22541). GAPDH load control was reused for illustrative purposes. Image collected and cropped by CiteAb from the following publication (frontiersin.org/article/10.3389/fimmu.2018.01767/full), licensed under a CC-BY license.
Immunohistochemistry-Frozen: ICAM-1/CD54 Antibody (1A29) [NBP2-22541] - Staining of frozen rat spleen with Mouse anti Rat CD54.
Flow Cytometry: ICAM-1/CD54 Antibody (1A29) [NBP2-22541] - Analysis of CD54 in C6 cells (green) compared to an isotype control (blue).
Applications for ICAM-1/CD54 Antibody (1A29)
Application
Recommended Usage
Flow Cytometry
1:10 - 1:1000
Immunocytochemistry/ Immunofluorescence
1 - 2 ug/ml
Immunohistochemistry
1:100
Immunohistochemistry-Frozen
1:100
Immunohistochemistry-Paraffin
1:100
Western Blot
1 - 3 ug/ml
Application Notes
Use in PLA reported in scientific literature (PMID:34572594). Use in Immunohistochemistry free floating, and Electron Microscopy reported in scientific literature (PMID: 8574890). .
Reviewed Applications
Read 1 review rated 4 using NBP2-22541 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2)
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: ICAM-1/CD54
Long Name
Intercellular Adhesion Molecule 1
Alternate Names
CD54, ICAM1
Entrez Gene IDs
15894 (Mouse)
Gene Symbol
ICAM1
Additional ICAM-1/CD54 Products
Product Documents for ICAM-1/CD54 Antibody (1A29)
Product Specific Notices for ICAM-1/CD54 Antibody (1A29)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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