MMP-14/MT1-MMP Antibody (6A11D1) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52512

Novus Biologicals, part of Bio-Techne
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NBP2-52512-0.025mg
NBP2-52512
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Key Product Details

Species Reactivity

Validated:

Human

Applications

CyTOF-ready, ELISA, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 6A11D1

Format

BSA Free

Concentration

1 mg/ml

View all MMP-14/MT1-MMP Antibodies »

Product Summary for MMP-14/MT1-MMP Antibody (6A11D1) - BSA Free

Immunogen

Purified recombinant fragment of human MMP-14/MT1-MMP (AA: 112-246) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

65.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MMP-14/MT1-MMP Antibody (6A11D1) - BSA Free

Western Blot: MMP-14/MT1-MMP Antibody (6A11D1) [NBP2-52512] - Analysis using MMP14 mAb against human MMP14 (AA: 112-246) recombinant protein. (Expected MW is 41.3 kDa)
Immunohistochemistry-Paraffin: MMP-14/MT1-MMP Antibody (6A11D1) [NBP2-52512] - Analysis of bladder cancer tissues using MMP14 mouse mAb with DAB staining.
Flow Cytometry: MMP-14/MT1-MMP Antibody (6A11D1) [NBP2-52512] - Analysis of HT1080 cells using MMP14 mouse mAb (green) and negative control (red).
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Applications for MMP-14/MT1-MMP Antibody (6A11D1) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunohistochemistry

1:200-1:1000

Immunohistochemistry-Paraffin

1:200-1:1000

Western Blot

1:500-1:2000
Application Notes
This antibody is Cytof ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MMP-14/MT1-MMP

The matrix metalloproteinase (MMP) family (approximately 25 members in mammals) has been implicated in extracellular matrix remodeling associated with embryonic development, cancer formation and progression, and various other physiological and pathological events. MMP-14 is a membrane-bound matrix metalloproteinase (MT-MMP) capable of mediating pericellular proteolysis of extracellular matrix components. MMP-14 is therefore thought to be an important molecular tool for cellular remodeling of the surrounding matrix (1). Gelatinase A (type-IV collagenase; M(r) 72,000) is produced by tumour stroma cells and is believed to be crucial for their invasion and metastasis, acting by degrading extracellular matrix macro-molecules such as type IV collagen. MMP-14 may thus trigger invasion by tumor cells by activating pro-gelatinase A on the tumor cell surface (2). Observations identify MT1-MMP as a tumor-derived growth factor that regulates proliferation by controlling cell geometry within the confines of the 3D extracellular matrix (ECM) (3).

Long Name

Matrix Metalloproteinase 14/Membrane Type 1 MMP

Alternate Names

MMP-X1, MMP14, MT-MMP1, MT1-MMP

Gene Symbol

MMP14

Additional MMP-14/MT1-MMP Products

Product Documents for MMP-14/MT1-MMP Antibody (6A11D1) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MMP-14/MT1-MMP Antibody (6A11D1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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