MMP-14/MT1-MMP Antibody (LEM-2/15) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-09002
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human
Applications
Block/Neutralize, ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG1 kappa Clone # LEM-2/15
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
The cyclic peptide possessing the sequence of residues 218-233 of the V-B-loop of MT1-MMP.
Specificity
This antibody binds to residues 218-233 of the V-B loop of MT1-MMP with a Kd of 2.32E10-9M.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for MMP-14/MT1-MMP Antibody (LEM-2/15) - Azide and BSA Free
Western Blot: MMP-14/MT1-MMP Antibody (LEM-2/15) [NBP3-09002] - A549 (A), HepG2 (B) and HeLa (C) cell lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric mouse IgG1 version of LEM-2/15 (NBP3-09002) at 0.3 ug/ml before detection using an anti-mouse secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The predicted running size for unprocessed MMP-14/MT1-MMP is 65.9 kDa. A signal peptide and propeptide are cleaved from MMP-14/MT1-MMP during post-translational processing. The processed form of MMP-14/MT1-MMP may be seen in all samples, and the unprocessed form in lanes B and C. NBP3-09002 successfully detected MMP-14/MT1-MMP in A549, HepG2 and HeLa cell lysates.
Applications for MMP-14/MT1-MMP Antibody (LEM-2/15) - Azide and BSA Free
Application
Recommended Usage
Block/Neutralize
Optimal dilutions of this antibody should be experimentally determined.
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody binds to residues 218-233 of the V-B loop of MT1-MMP which is a mediator of cell migration and is frequently involved in malignancy of tumours by promoting metastasis. The antibody acts as a non-competitive inhibitor of the receptor.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: MMP-14/MT1-MMP
Long Name
Matrix Metalloproteinase 14/Membrane Type 1 MMP
Alternate Names
MMP-X1, MMP14, MT-MMP1, MT1-MMP
Gene Symbol
MMP14
Additional MMP-14/MT1-MMP Products
Product Documents for MMP-14/MT1-MMP Antibody (LEM-2/15) - Azide and BSA Free
Product Specific Notices for MMP-14/MT1-MMP Antibody (LEM-2/15) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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