Mouse CD90/Thy1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB7335
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
CyTOF-ready, Flow Cytometry, Western Blot
Cited:
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 778053
Product Specifications
Specificity
Detects mouse CD90/Thy1 in ELISAs. In direct ELISAs, no cross-reactivity with recombinant
human CD90/Thy1 is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Mouse CD90/Thy1 Antibody
Detection of Mouse CD90/Thy1 by Western Blot.
Western blot shows lysates of mouse brain tissue and mouse thymus tissue. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Mouse CD90/Thy1 Monoclonal Antibody (Catalog # MAB7335) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). Specific bands were detected for CD90/Thy1 at approximately 25-30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of CD90/Thy1 in Mouse Splenocytes by Flow Cytometry.
Mouse splenocytes were stained with Rat Anti-Mouse CD3 PE-conjugated Monoclonal Antibody (Catalog # FAB4841P) and either (A) Rat Anti-Mouse CD90/Thy1 Monoclonal Antibody (Catalog # MAB7335) or (B) Rat IgG2AIsotype Control (Catalog # MAB006) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).Applications for Mouse CD90/Thy1 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: Mouse splenocytes
Sample: Mouse splenocytes
Western Blot
0.5 µg/mL
Sample: Mouse brain tissue and mouse thymus tissue
Sample: Mouse brain tissue and mouse thymus tissue
Formulation, Preparation, and Storage
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Background: CD90/Thy1
Alternate Names
CD90, Thy1
Gene Symbol
THY1
UniProt
Additional CD90/Thy1 Products
Product Documents for Mouse CD90/Thy1 Antibody
Product Specific Notices for Mouse CD90/Thy1 Antibody
For research use only
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