Mouse ICAM-1/CD54 Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF796
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
ELISA Detection (Matched Antibody Pair), Immunohistochemistry, Western Blot
Cited:
Luminex Development
Label
Biotin
Antibody Source
Polyclonal Goat IgG
Product Summary for Mouse ICAM-1/CD54 Biotinylated Antibody
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse ICAM‑1/CD54
Gln28-Asn485
Accession # Q3U8M7
Gln28-Asn485
Accession # Q3U8M7
Specificity
Detects mouse ICAM‑1/CD54 in ELISAs and Western blots. In sandwich ELISAs, less than 0.3% cross‑reactivity with recombinant human ICAM-1, recombinant rat ICAM-1, and recombinant mouse ICAM-2 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Mouse ICAM-1/CD54 Biotinylated Antibody
ICAM‑1/CD54 in Human Kidney.
ICAM-1/CD54 was detected in immersion fixed paraffin-embedded sections of human kidney using Goat Anti-Mouse ICAM-1/CD54 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF796) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cell membrane in convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Mouse ICAM-1/CD54 Biotinylated Antibody
Application
Recommended Usage
Immunohistochemistry
2-10 µg/mL
Sample: Perfusion fixed frozen sections of mouse thymus and immersion fixed paraffin-embedded sections of human kidney
Sample: Perfusion fixed frozen sections of mouse thymus and immersion fixed paraffin-embedded sections of human kidney
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse ICAM-1/CD54 Fc Chimera (Catalog # 796-IC)
Sample: Recombinant Mouse ICAM-1/CD54 Fc Chimera (Catalog # 796-IC)
Mouse ICAM-1/CD54 Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 3 reviews rated 4.3 using BAF796 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ICAM-1/CD54
Intercellular Adhesion Molecule-1 (ICAM-1, CD54) binds the leukocyte integrins LFA-1 and Mac-1. ICAM-1 expression is weak on leukocytes, epithelial and resting endothelial cells, as well as some other cell types, but expression can be stimulated by IFN-gamma, TNF-alpha, IL-1 beta and LPS. Mouse and human ICAM-1 share approximately 54% amino acid identity.
Soluble ICAM-1 is found in a biologically active form in serum, probably as a result of proteolytic cleavage from the cell surface, and is elevated in patients with various inflammatory syndromes such as septic shock, leukocyte adhesion deficiency syndrome (LAD), cancer and transplantation.
References
- Pigott, R. and C. Power (1993) in The Adhesion Molecule Facts Book. Academic Press, p. 74.
- Siu, G. et al. (1989) J. Immunol. 143:3813.
- Ballantyne, C.M. et al. (1989) Nuc. Acid. Res. 17:5853.
Long Name
Intercellular Adhesion Molecule 1
Alternate Names
CD54, ICAM1
Gene Symbol
ICAM1
UniProt
Additional ICAM-1/CD54 Products
Product Documents for Mouse ICAM-1/CD54 Biotinylated Antibody
Product Specific Notices for Mouse ICAM-1/CD54 Biotinylated Antibody
For research use only
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