Detects rat CXCR2/IL-8 RB in direct ELISAs. In Flow Cytometry, stains HEK293 cells transfected with rat CXCR2/IL-8 RB, but does not stain non‑transfected cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Scientific Data Images for Rat CXCR2/IL-8RB Antibody

Detection of CXCR2/IL‑8 RB in Rat Blood Granulocytes by Flow Cytometry.

Rat peripheral blood granulocytes were stained with Mouse Anti-Rat CXCR2/IL-8 RB Monoclonal Antibody (Catalog # MAB8110, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Detection of CXCR2/IL‑8 RB in HEK293 Human Cell Line Transfected with Rat CXCR2/IL-8 RB and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with either (A) rat CXCR2/IL-8 RB or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Rat CXCR2/IL-8 RB Monoclonal Antibody (Catalog # MAB8110) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). Quadrant markers were set based on control antibody staining (Catalog # MAB004). View our protocol for Staining Membrane-associated Proteins.

Applications for Rat CXCR2/IL-8RB Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: Rat peripheral blood granulocytes and HEK293 human embryonic kidney cell line transfected with rat CXCR2/IL-8 RB and eGFP

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: CXCR2/IL-8RB

CXCR2 is an approximately 40 kDa 7-transmembrane domain receptor for the ELR+ chemokines CXCL1, 2, 3, 5, 6, 7, 8 and MIF. CXCR2 is expressed on neutrophils, monocytes, eosinophils, basophils, mast cells, T cells, oligodendrocytes, airway smooth muscle cells, and vascular endothelial cells. It is additionally upregulated in several cancers. CXCR2 can associate into homodimers or heterodimers with CXCR1, CXCR4, CD74, or the delta Opioid Receptor. CXCR2 plays an important role in attracting immune cells to sites of inflammation followed by their adhesion and extravasation. It is also involved in angiogenesis, the development of inflammatory disorders, and cancer. Rat CXCR2 shares 71% and 86% amino acid sequence identity with human and mouse CXCR2, respectively.

Long Name

Interleukin 8 Receptor B

Alternate Names

CD182, CDw128b, CMKAR2, CXCR-2, IL-8 RB, IL8RB

Entrez Gene IDs

3579 (Human); 12765 (Mouse); 29385 (Rat)

Gene Symbol

CXCR2

UniProt

P35407 (Rat)

Additional CXCR2/IL-8RB Products

Product Documents for Rat CXCR2/IL-8RB Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Rat CXCR2/IL-8RB Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Rat CXCR2/IL-8RB Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB8110

Key Product Details

Species Reactivity

Validated:

Applications

Label

Antibody Source

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Product Summary for Rat CXCR2/IL-8RB Antibody

Specificity