V5 Epitope Tag Antibody - BSA Free

Western Blot: V5 Epitope Tag Antibody [NB600-381] - NP-40 whole cell lysate (10 ug) from wild-type HEK293 cells (lane 1) or HEK293 cells expressing V5 tagged Nicastrin (lane 2). NB600-381 was used at 0.2 ug/mL (1:5000).

Western Blot: V5 Epitope Tag Antibody [NB600-381] - Analysis using the HRP conjugate of NB600-381. Detection of 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.2 ug/mL (1:5,000).

Immunocytochemistry/Immunofluorescence: V5 Epitope Tag Antibody [NB600-381] - HEK293 cells transiently expressing V5-tagged mutant PFN1 stained with V5 Epitope Tag antibody (red). ICC/IF image submitted by a verified customer review.

Immunocytochemistry/Immunofluorescence: V5 Epitope Tag Antibody [NB600-381] - Drosophila adult midgut expressing a V5-tagged protein (magenta) using the GAL4/UAS system. Nuclei are shown in green. pAb dilution of 1:500. ICC/IF image submitted by a verified customer review.

Immunoprecipitation: V5 Epitope Tag Antibody [NB600-381] - HEK293T were transfected with V5-KU86 plasmid, followed by V5 antibody IP then Western blot with v5 antibody (NB600-381). Primary antibody at 1:1000, 4C, overnight. IP/WB image submitted by a verified customer review.

Immunocytochemistry/Immunofluorescence: V5 Epitope Tag Antibody - BSA Free [NB600-381] -

Ft and Ds show mutually dependent stable fractions at junctions. F) Sub-apical confocal sections of pre-pupal wing show V5-Ft previously at the cell surface is seen in discrete intracellular puncta at t = 20, where it co-localises with early endosome marker Hrs (magenta) (F'), confirming antibody internalisation rather than antibody drop off. FRAP analysis of Ds-EGFP in ftG-rv clones (G) and Ft-EGFP in a dsUA071 background (H) resulted in more rapid and increased protein recovery. Dashed lines denote 95% confidence intervals. As recovery did not reach 100%, a small stable population could remain. Also see Figure 2-figure supplement 4 for FRAP graphs with individual data points. Re-bleaching experiments suggest that this is not an experimental artefact (see Figure 2-figure supplement 5). Live images of Ds-EGFP (I) and Ds-EGFP with a ftG-rv clone (I'; blue dots indicate first row of mutant cells) show a loss of puncta and diffuse distribution of Ds-EGFP when Ft is not present. Similar distribution of Ft-EGFP (J) is seen in a dsUA071 mutant (J'). Image collected and cropped by CiteAb from the following publication (https://elifesciences.org/articles/05789), licensed under a CC-BY licence.

Immunocytochemistry/Immunofluorescence: V5 Epitope Tag Antibody - BSA Free [NB600-381] -

IL-6 impairs intracellular killing of M. tuberculosis through altered LD homeostasis.(d) ATG2A-V5 overexpression reduces intracellular neutral lipids in THP-1 cells. Scale bars, 10 um. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/ncomms14642), licensed under a CC-BY licence.

Western Blot: V5 Epitope Tag Antibody - BSA Free [NB600-381] -

(a) Light and fluorescent micrographs of stable HN33.11 cells expressing (A)-GFP or (B)-GFP, respectively. Results from two stable clones of each transfection pool are shown. Cells are treated with vehicle (Dox-) or 1 ug/mL Dox (Dox+). (b) Western blot of homogenates of stable clones showing endogenous production of full-length p75NTR (FLp75), Dox-induced, but "leaky" production of (A) and (B) and of the V5 tag. Control values for each clone do not differ significantly between GFP(-) and GFP(+) cells (Student's t-test). ***P 0.001 relative to GFP(-) cells. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/21904642), licensed under a CC-BY licence.