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V5 Epitope Tag Antibody [Biotin]

Novus Biologicals, part of Bio-Techne | Catalog # NB600-381B

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Epitope Tag

Applications

Validated:

ELISA, Immunocytochemistry/Immunofluorescence, Western Blot

Cited:

ELISA

Label

Biotin

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

Product Specifications

Immunogen

Rabbits were immunized with a synthetic peptide representing amino acid residues 95 to 108 of RNA polymerase alpha subunit of simion virus 5 conjugated to KLH.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 29017057).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for V5 Epitope Tag Antibody [Biotin]

Western Blot: V5 Epitope Tag Antibody [Biotin] [NB600-381B] - 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.04 ug/ml (1:25,000). Detection: Streptavidin-HRP (0.1 ug/ml) and chemiluminescence with an exposure time of 10 seconds.

Applications for V5 Epitope Tag Antibody [Biotin]

Application
Recommended Usage

ELISA

1:100-1:2000

Western Blot

1:10000-1:100000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS and 0.2% BSA

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: V5 Epitope Tag

The V5 epitope tag is commonly engineered onto the N- or C- terminus of a protein of interest so that the tagged protein can be analyzed and visualized using immunochemical methods. The recognized V5 epitope represents amino acid residues 95 to 108, GKPIPNPLLGLDST, of RNA polymerase alpha subunit of simian virus 5.

Alternate Names

GKPIPNPLLGLDST Epitope Tag, Parainfluenza virus 5 V/P, Paramyxovirus SV5

Additional V5 Epitope Tag Products

Product Documents for V5 Epitope Tag Antibody [Biotin]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for V5 Epitope Tag Antibody [Biotin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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