Key Product Details

Species Reactivity

Rabbit

Applications

Immunoprecipitation, SDS-Page

Label

Agarose

Antibody Source

Rabbit IgG

Concentration

Please contact technical services for concentration.

View all IgG Isotype Controls »

Product Summary for Rabbit IgG Isotype Control [Agarose]

Host

Rabbit

Isotype

IgG

Description

Store vial at 4C prior to opening. DO NOT FREEZE.

This product is normal Rabbit IgG coupled to activated agarose. Assay by immunoelectrophoresis prior to coupling to the beads.

Scientific Data Images for Rabbit IgG Isotype Control [Agarose]

SDS-Page: Rabbit IgG Isotype Control [Agarose Immobilized] [NBP1-97118] - Lane M: 3 uL Opal Prestained Marker. Lane 1: Reduced Rabbit IgG Whole Molecule Agarose Conjugated. Lane 2: Reduced Rabbit IgG F(ab) Fragment. Lane 3: Reduced Rabbit IgG F(c) Fragment. Lane 4: Reduced Rabbit IgM Whole Molecule. Load: 1 ug for F(ab) and F(c); 1.2 ug for IgG and IgM.

Applications for Rabbit IgG Isotype Control [Agarose]

Application
Recommended Usage

Immunoprecipitation

1:10 - 1:500

SDS-Page

1:100 - 1:2000
Application Notes
This product is suitable for immunoprecipitation and Immunoaffinity purifications.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Multi-step

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% Sodium Azide

Concentration

Please contact technical services for concentration.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: IgG

IgG is a monomeric immunoglobulin, built of two heavy chains gamma and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, thereby providing protection to the fetus in its first weeks of life before its own immune system has developed. It can bind to many kinds of pathogens, for example viruses, bacteria, and fungi, and protects the body against them by complement activation (classic pathway), opsonization for phagocytosis and neutralisation of their toxins. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%).

Long Name

Immunoglobulin G

Alternate Names

Immunoglobulin G, ImmunoglobulinG

Gene Symbol

IGHG1

Additional IgG Products

Product Documents for Rabbit IgG Isotype Control [Agarose]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Rabbit IgG Isotype Control [Agarose]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

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