Rabbit IgG Isotype Control
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-36463
Conjugate
Catalog #
Key Product Details
Species Reactivity
Rabbit
Applications
Chromatin Immunoprecipitation (ChIP), ELISA, Flow (Intracellular), Immunohistochemistry, SDS-Page, Western Blot
Label
Unconjugated
Antibody Source
Rabbit IgG
Format
Purified
Concentration
Please contact technical services for concentration.
Product Summary for Rabbit IgG Isotype Control
Host
Rabbit
Isotype
IgG
Description
This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit IgG and anti-Rabbit Serum.
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for Rabbit IgG Isotype Control
Western Blot: Rabbit IgG Isotype Control [NBP2-36463] - Lane M: 3 ul Molecular Ladder. Lane 1: Rabbit IgG whole molecule (NBP2-36463). Lane 2: Rabbit IgG F(ab) Fragment (NBP1-97016). Lane 3: Rabbit IgG F(c) Fragment (NBP1-96784). Lane 4: Rabbit IgM Whole Molecule (NBP1-96968). Lane 5: Normal Rabbit Serum. All samples were reduced. Load: 50 ng per lane. Blocked in blocking buffer for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (GOAT) Antibody (Min X Bv, Ch, Gt, GP, Ham, Hs, Hu, Ms, Rt & Sh Serum Proteins. (NBP1-72732) 1:1,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 in blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.
Flow (Intracellular): Rabbit IgG Isotype Control [NBP2-36463] - Intracellular staining for GLI1, GLI2 and GLI3 in untreated human B cell lines (BCWM.1, MWCL-1 and RPCI-WM1) using a secondary anti-rabbit IgG PE conjugated antibody. Image courtesy of Sherine Elsawa, Northern Illinois University.
SDS-Page: Rabbit IgG Isotype Control [NBP2-36463] - Lane 1: Non-reduced Rabbit IgG Whole Molecule. Lane 2: 5uL OPAL Pre-stained Marker. Lane 3: Reduced Rabbit IgG Whole Molecule. Load: 1ug per lane. Predicted/Observed size: Non-reduced at 150-170 kDa , Reduced at 55, 25 kDa.
Applications for Rabbit IgG Isotype Control
Application
Recommended Usage
Chromatin Immunoprecipitation (ChIP)
Optimal dilutions of this antibody should be experimentally determined.
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Flow (Intracellular)
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
SDS-Page
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This product has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.
Use in ChIP reported in scientific literature (PMID:34335948).
Use in ChIP reported in scientific literature (PMID:34335948).
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Multi-step
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Format
Purified
Preservative
0.01% Sodium Azide
Concentration
Please contact technical services for concentration.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: IgG
Long Name
Immunoglobulin G
Alternate Names
Immunoglobulin G, ImmunoglobulinG
Gene Symbol
IGHG1
Additional IgG Products
Product Documents for Rabbit IgG Isotype Control
Product Specific Notices for Rabbit IgG Isotype Control
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.
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