Recombinant Human IL-1 beta/IL-1F2, Animal-Free Protein

R&D Systems, part of Bio-Techne | Catalog # AFL201

Intended for preclinical researchers who may transition to GMP IL-1beta for their clinical work
R&D Systems, part of Bio-Techne
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AFL201-025
AFL201
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Key Product Details

Learn more about Animal-Free Recombinant Proteins

Source

E. coli

Accession #

NP_000567.1

Conjugate

Unconjugated

Applications

Bioactivity

View all IL-1 beta/IL-1F2 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human IL-1 beta/IL-1F2 protein
Ala117-Ser269
Produced using non-animal reagents in an animal-free laboratory.

Purity

>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala117 & Pro118

Predicted Molecular Mass

17 kDa

Activity

Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272.
The ED50 for this effect is <12 pg/mL. 

The specific activity of Recombinant Human IL-1 beta is >5.0 x 107 IU/mg, which is calibrated against recombinant human IL-1 beta WHO International Standard (NIBSC code: 86/680).

Formulation, Preparation and Storage

AFL201
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 0.2 mg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 12 months, 2 to 8 °C under sterile conditions after reconstitution.

Background: IL-1 beta/IL-1F2

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 21% amino acid (aa) identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL‑1 R3/IL‑1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI (1‑4). The human IL-1 beta cDNA encodes a 269 aa precursor. A 116 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta-converting enzyme (Caspase-1/ICE) to generate the active cytokine (5‑7). The 17 kDa mature human IL-1 beta shares 96% aa sequence identity with rhesus and 67%‑78% with canine, cotton rat, equine, feline, mouse, porcine, and rat IL‑1 beta.

References

  1. Allan, S.M. et al. (2005) Nat. Rev. Immunol. 5:629.
  2. Boraschi, D. and A. Tagliabue (2006) Vitam. Horm. 74:229.
  3. Kornman, K.S. (2006) Am. J. Clin. Nutr. 83:475S.
  4. Isoda, K. and F. Ohsuzu (2006) J. Atheroscler. Thromb. 13:21.
  5. March, C.J. et al. (1985) Nature 315:641.
  6. Auron, P.E. et al. (1984) Proc. Natl. Acad. Sci. 81:7907.
  7. Martinon, F. and J. Tschopp (2007) Cell Death Differ. 14:10.

Long Name

Interleukin 1 beta

Alternate Names

IL-1b, IL-1F2, IL1 beta, IL1B

Entrez Gene IDs

3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)

Gene Symbol

IL1B

UniProt

NP_000567.1

Additional IL-1 beta/IL-1F2 Products

Product Documents for Recombinant Human IL-1 beta/IL-1F2, Animal-Free Protein

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Manufacturing Specifications

Animal-Free Manufacturing Conditions
Our dedicated controlled-access animal-free laboratories ensure that at no point in production are the products exposed to potential contamination by animal components or byproducts. Every stage of manufacturing is conducted in compliance with R&D Systems' stringent Standard Operating Procedures (SOPs). Production and purification procedures use equipment and media that are confirmed animal-free.  

 Production

  • All molecular biology procedures use animal-free media and dedicated labware.
  • Dedicated fermentors are utilized in committed animal-free areas.

Purification

  • Protein purification columns are animal-free.
  • Bulk proteins are filtered using animal-free filters.
  • Purified proteins are stored in animal-free containers in a dedicated cold storage room.

    Quality Assurance

    • Low Endotoxin Level.
    • No impairment of biological activity.
    • High quality product obtained under stringent conditions.
    • For ex vivo research or bioproduction, additional documentation can be provided.

    Please read our complete Animal-Free Statement

    Product Specific Notices for Recombinant Human IL-1 beta/IL-1F2, Animal-Free Protein

    This product is covered by one or more of the following U.S. patents: 5,681,933 and 5,474,899.

    For research use or further manufacturing only

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