Flow Cytometry Solutions for CAR Cell Therapy
Easily Identify and Phenotype CAR-T Cells by Flow Cytometry
After transduction of T cells or NK cells with the chimeric antigen receptor (CAR), it is important to assess the degree of transduction (purity) as well as understand the phenotype of transduced cells (potency). Bio-Techne’s fluorescent conjugated antibodies and Fluorokine™ proteins allow you to assess this in one simple staining step.
CAR Cell Panel
| Marker | Clone | Fluorochrome | Catalog Number |
|---|---|---|---|
| CD14 |
134620 |
Alexa Fluor® 700 |
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| CD45 | 2D1 | PerCP | |
| CD56/NCAM-1 | 2524C | PE | |
| CD8 | 37006 |
Alexa Fluor® 405 |
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CD4 |
- |
PE-Cy7TM |
Available from Novus Biologicals |
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CD62L/L-Selectin |
4G8 |
Alexa Fluor® 594 |
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Human CD19 Fc Chimera |
- |
Atto 647 |
FluorokinesTM Can be Used with Fluorochrome-conjugated Antibodies to Characterize CD19-CAR-T Cells by Multi-Color Flow Cytometry. Human PBMC CD4+CD8+ T cells transduced with a hCD19-CAR were stained with the following panel of monoclonal antibodies: Alexa Fluor® 700-conjugated Mouse Anti-Human CD14 (Catalog # FAB3832N), PerCP-conjugated Mouse Anti-Human CD45 (Catalog # FAB1430C), PE-conjugated Rabbit Anti-Human CD56/NCAM-1 (Catalog # FAB24086P), Alexa Fluor® 405-conjugated Mouse Anti-Human CD8 (Catalog # FAB1509V), PE-Cy7-CD4, and Alexa Fluor® 594-conjugated Mouse Anti-Human CD62L/L-Selectin (Catalog # FAB9787T), along with the Fluorokine, Recombinant Human CD19 Fc Chimera Atto 647N Protein (Catalog # ATM9269) and detected by flow cytometry. Cells were initially gated on singlets and live cells.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
Evaluating CAR Expression on CAR-T Cells Using FluorokinesTM
Evaluating CAR Expression on CAR-T Cells Using FluorokinesTM
One of the current challenges in the field of CAR-T cell therapy is the lack of a simple assay to easily detect and quantify CAR expression following T cell transduction. Read this application note to learn about the advantages of using R&D SystemsTM Fluorokines fluorescent-labeled proteins to directly stain and detect CAR+ cells by flow cytometry.
The CAR ToolKit: Progress and Challenges in CAR-T/NK Cell R&D and Mfg
The CAR ToolKit: Progress and Challenges in CAR-T/NK Cell R&D and Mfg
With numerous clinical trials underway and multiple CAR-T cell products now approved, solving the current obstacles when manufacturing CAR-T/NK cell products continues to be a pressing issue for the cellular immunotherapy field. Watch this webinar, hosted by BioInsights, to learn about the current challenges, latest progress, and innovation facing CAR-T and CAR-NK cell development.
Flow Cytometry Panel Builder Tool
Flow Cytometry Panel Builder Tool
The Bio-Techne Panel Builder tool is designed to prevent selection of incompatible fluorochromes by blocking channels with excessive spectral overlap. Save time and reduce costly mistakes by quickly finding compatible reagents for multicolor flow cytometry experiments using the Panel Builder Tool.
Fluorescent Conjugated Proteins for CAR Detection
The ability to evaluate the expression of a chimeric antigen receptor (CAR) following T cell or natural killer (NK) cell transduction is a critical step in the production of CAR-T or CAR-NK cells. FluorokineTM proteins are directly conjugated with fluorescent molecules like Alexa Fluor® 647, Alexa Fluor® 488, Atto 488, and Atto 647. Fluorokines are highly specific, reduce processing time, and eliminate background staining that may occur by indirect detection of the CAR using an epitope-tagged target antigen and a fluorophore-labeled secondary antibody.
Fluorescent-Labeled Proteins for CAR-T or CAR-NK Cell Detection
Custom Fluorokine Solutions
Unable to find the fluorescent-labeled protein that you need? Contact our trusted Custom Protein Services team can work with you to create a customized protein solution to meet your specific research needs.
Flow Cytometry Validated Antibodies
At Bio-Techne, we know the importance of quality. Our flow cytometry antibodies are backed by our 100% Guarantee so you can have confidence in your purchase.
Antibodies to Phenotype T Cells
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Memory T Cell Markers |
T Cell Exhaustion Markers |
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T Cell Activation Markers |
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Antibodies to Phenotype NK Cells
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NK Cell Subsets |
NCR family |
KIR family |
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The Fastest Way to a Single Cell
Hana and Pala from Namocell, now a Bio-Techne brand help you sort and dispense cells into 96-well or 384-well plates or 2mL tubes on your own bench. Three patented sorting modes:
- Single cell sorting mode dispenses single cells one-cell-per-well.
- Enrichment mode isolates rare cells from extremely large samples.
- Bulk sorting mode sorts tens of thousands cells in bulk.
Fluorescent Dyes
Cell Viability Dyes
Non-nuclear Dyes
- DRAQ9
- MitoBrilliant™ dyes for mitochondrial staining
Quality Control and Standardization Beads
Set yourself up for successful flow cytometry experiments by using our Calibration, Compensation, Cell Counting, and Equivalent Reference Fluorochromes (ERF) beads.
Complete Your CAR T Cell or CAR NK Cell Workflow
Bio-Techne is here to be your partner through every stage of the CAR T Cell and CAR NK Cell Therapy workflow, from cell isolation through in vivo monitoring.
Learn more about T Cell Manufacturing and NK Cell Manufacturing with Bio-Techne.
Immune Cell Culture and Expansion
Immune Cell Culture and Expansion
We offer a wide selection of high-quality cell culture media, supplements, and recombinant proteins for improved survival and expansion of different immune cell types.
GMP Proteins for Cell and Gene Therapy
GMP Proteins for Cell and Gene Therapy
Discover the latest GMP and animal-free RUO proteins available from our state-of-the-art animal-free GMP manufacturing facility located in Saint Paul, Minnesota.
Scale Up with ScaleReady
Scale Up with ScaleReady
Advance your cultures with an innovative cell expansion platform that alleviates scale-up challenges. Our partners at ScaleReady offer G-Rex® 6M well plates and closed-system bioreactors that allow the same protocols to be used from early research through commercial scale up, dramatically simplifying transition to GMP manufacturing.
Transition to Animal-Free and GMP
Transition to Animal-Free and GMP
We understand the need for adopting increasingly well-defined raw materials. Regardless of where your program is in this trajectory, you can rely on us to provide multiple grades of key materials – with flexibility and customization to fit your process.