Key Product Details

Species Reactivity

Validated:

Mouse

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry, Immunohistochemistry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rat IgG2B Clone # 247506R

View all CXCR4 Antibodies »

Product Summary for Mouse CXCR4 Antibody

Specificity

Detects mouse CXCR4 transfectants. Does not stain irrelevant transfectants.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Mouse CXCR4 Antibody

Detection of CXCR4 antibody in HEK293 Human Cell Line Transfected with Mouse CXCR4 and eGFP antibody by Flow Cytometry.

Detection of CXCR4 in HEK293 Human Cell Line Transfected with Mouse CXCR4 and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with mouse CXCR4 and eGFP was stained with (A) Rat Anti-Mouse CXCR4 Monoclonal Antibody (Catalog # MAB21651R) or (B) Rat IgG2B isotype control antibody (Catalog # MAB0061) followed by APC-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.
Detection of CXCR4 antibody in Mouse Thymocytes antibody by Flow Cytometry.

Detection of CXCR4 in Mouse Thymocytes by Flow Cytometry.

Mouse thymocytes were stained with Rat Anti-Mouse CXCR4 Monoclonal Antibody (Catalog # MAB21651R, filled histogram) or Rat IgG2B isotype control antibody (Catalog # MAB0061, open histogram) followed by Goat anti-Rat IgG PE-conjugated Secondary Antibody (Catalog # F0105B). View our protocol for Staining Membrane-associated Proteins.
CXCR4 antibody in Mouse Splenocytes by Immunocytochemistry (ICC).

CXCR4 in Mouse Splenocytes.

CXCR4 was detected in immersion fixed mouse splenocytes using Rat Anti-Mouse CXCR4 Monoclonal Antibody (Catalog # MAB21651R) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
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Applications for Mouse CXCR4 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Mouse thymocytes and HEK293 Human Cell Line Transfected with Mouse CXCR4 and eGFP

Immunocytochemistry

1-25 µg/mL
Sample: Immersion fixed mouse splenocytes

Immunohistochemistry

5-25 µg/mL
Sample: Perfusion fixed frozen sections of mouse spleen and perfusion fixed frozen sections of mouse testis
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: CXCR4

CXCR4, also known as CD184, is a G-protein-linked seven transmembrane spanning receptor that binds stromal cell-derived factor-1 (SDF-1). CXCR4 acts as a co-factor for T-cell tropic HIV-1 and -2 viral entry into cells. While primarily a membrane protein, CXCR4 undergoes trafficking and internalization in response to stimulation with phorbol esters and ligand (1). Cytoplasmic and nuclear localization of CXCR4 has been observed in colorectal and renal carcinomas (2,3) and it has been used as the basis of prognosis and metastatic state (3,4,5).

References

  1. Orsini, M.J. et al. (1999) J. Biol. Chem. 274:31076.
  2. Zagzag, D. et al. (2005) Cancer Res. 65:6178.
  3. Speetjens, F.M. et al. (2009) Cancer Microenvironment 2:1.
  4. Wang, L. et al. (2009) Oncology Reports 22:1333.
  5. Amara, S. et al. (2015) Cancer Biomark. 15:869.

Long Name

C-X-C Motif Chemokine Receptor 4

Alternate Names

CD184, D2S201E, FB22, Fusin, HM89, HSY3RR, LAP-3, LAP3, LCR1, LESTR, NPY3R, NPYRL, NPYY3R, WHIMS

Entrez Gene IDs

7852 (Human); 12767 (Mouse); 60628 (Rat); 483900 (Canine); 493676 (Feline)

Gene Symbol

CXCR4

UniProt

P70658 (Mouse)

Additional CXCR4 Products

Product Documents for Mouse CXCR4 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse CXCR4 Antibody

For research use only

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