Purmorphamine

Name: Purmorphamine
Brand: Tocris Bioscience
SKU: 4551
Rating: 5 (3 reviews)

Tocris Bioscience, part of Bio-Techne | Catalog # 4551

(3)

Citations (105)

COA / SDS

Smo receptor agonist

Key Product Details

Description:	Smo receptor agonist
Chemical Name:	9-Cyclohexyl-N-[4-(4-morpholinyl)phenyl]-2-(1-naphthalenyloxy)-9H-purin-6-amine
Purity:	≥98% (HPLC)
Molecular Weight:	520.62

View Full Technical Details for Purmorphamine

Technical Data
Citations / Reviews (3)

Biological Activity for Purmorphamine

Purmorphamine is a smoothened (Smo) receptor agonist (EC₅₀ ~ 1 μM) and activates the Hedgehog pathway. Purmorphamine induces osteogenesis in mouse mesenchymal progenitor cells (C3H10T1/2). When combined with BMP-4, Purmorphamine can transdifferentiate pre-adipocytes (3T3-L1) and myoblasts (C2C12) into osteoblasts. Purmorphamine induces differentiation of multipotent mesenchymal progenitor cells into osteoblasts, and of spinal motor neurons from pluripotent human stem cells, and protects dopaminergic neurons in a mouse model of Parkinson's disease.

Compound Libraries for Purmorphamine

Purmorphamine is also offered as part of the Tocriscreen 2.0 Max and Tocriscreen Stem Cell Library. Find out more about compound libraries available from Tocris.

Scientific Data Examples for Purmorphamine

	Applications of Purmorphamine in Neurons Derived from hiPSCs Dopaminergic neurons derived from human induced pluripotent stem cells (hiPSC) using SB 431542 (Catalog # 1614, Tocris), CHIR 99021 (Catalog # 4423, Tocris), DAPT (Catalog # 2634, Tocris) and Purmorphamine. Cells cultured in Neuronal Media supplemented with TGF-3 (Catalog # 8420-B3, R&D Systems), cAMP, GDNF (Catalog # 212-GD, R&D Systems), BDNF (Catalog # 248-BDB, R&D Systems), N21-MAX (Catalog # AR008, R&D Systems). Differentiation shown at days 55 (left) and 62 (right). Images courtesy of Kevin Flynn, Bio-Techne.

Technical Data for Purmorphamine

M. Wt	520.62
Formula	C₃₁H₃₂N₆O₂
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	483367-10-8
PubChem ID	5284329
InChi Key	FYBHCRQFSFYWPY-UHFFFAOYSA-N
Smiles	C1(OC4=CC=CC5=C4C=CC=C5)=NC(NC3=CC=C(N6CCOCC6)C=C3)=C2C(N(C7CCCCC7)C=N2)=N1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Purmorphamine

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	52.06	100

Preparing Stock Solutions for Purmorphamine

The following data is based on the product molecular weight 520.62.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.92 mL	9.60 mL	19.21 mL
5 mM	0.38 mL	1.92 mL	3.84 mL
10 mM	0.19 mL	0.96 mL	1.92 mL
50 mM	0.04 mL	0.19 mL	0.38 mL

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References for Purmorphamine

References are publications that support the biological activity of the product.

Sinha Purmorphamine activates the Hedgehog pathway by targeting Smoothened. Nat.Chem.Biol. 2006 PMID: 16408088
Wu A small molecule with osteogenesis-inducing activity in multipotent mesenchymal progenitor cells. J.Am.Chem.Soc. 2002 PMID: 12465946
Wu Purmorphamine induces osteogenesis by activation of the hedgehog signaling pathway. Chem.Biol. 2004 PMID: 15380183